Construct: ORF TRCN0000466428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006396.1_s317c1
- Derived from:
- ccsbBroadEn_13232
- DNA Barcode:
- TCGGCCCCACTTCGGGATTGCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZSCAN29 (146050)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466428
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146050 | ZSCAN29 | zinc finger and SCAN domain... | NM_001372080.1 | 54.2% | 54.2% | 1_3delATG;523_1689del |
| 2 | human | 146050 | ZSCAN29 | zinc finger and SCAN domain... | NM_152455.3 | 54.2% | 54.2% | 1_3delATG;523_1689del |
| 3 | human | 146050 | ZSCAN29 | zinc finger and SCAN domain... | XR_001751103.2 | 48.5% | 1_138del;658_1929del;2797_2852del | |
| 4 | mouse | 99334 | Zscan29 | zinc finger SCAN domains 29 | NM_001290819.1 | 47.5% | 45% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1455
- ORF length:
- 1386
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaaatca gctctaagag agaatggcac taactctgag accttccgac 121 agcgtttcag gagattccat taccaggagg tggctgggcc gcgggaggct ttcagccaac 181 tctgggaact ttgctgtcgg tggctaaggc cggaggtgcg caccaaggag cagattgtag 241 aactgttggt gctagagcag ttcctgaccg tcttacctgg ggagatccag aattgggtac 301 aggaacaatg tccagaaaat ggagaggagg cagtgactct cgtggaagat ttagaaagag 361 agcctggaag acctagatct tcggtcacag tctctgtgaa ggggcaggaa gtgcgcttgg 421 agaagatgac acccccgaaa tcatcacaag agttattaag tgttcggcag gagtcagtgg 481 aaccccagcc caggggtgta cccaagaaag agagggcaag aagcccagac ctgggaccac 541 aggagcagat gaacccaaag gagaagctca aaccttttca aaggagcggt tttgaagctg 601 gatttgagaa tgaagataat tcaaaacggg atatttctga ggaagtacaa ctgcatagga 661 cattacttgc aagatctgaa aggaaaattc cccggtatct tcatcagggt aaaggcaatg 721 agagtgactg tagatcagga agacagtggg caaagacctc aggggagaaa agaggaaaac 781 tgacactccc ggagaagagc ttaagtgaag tcctaagtca acagagacct tgcttgggag 841 agagacccta taaatatctc aaatacagca aaagctttgg tccaaactcc cttctcatgc 901 atcaggtatc ccaccaggtg gaaaatccat ataaatgtgc tgattgtggg aaaagcttca 961 gtcggagtgc acgactcatt agacaccgga gaatccacac tggagagaaa ccttataaat 1021 gtcttgactg tggaaaaagt ttccgtgaca gttcaaattt catcacccat aggagaatcc 1081 acacaggaga gaaaccttat caatgtggtG AGTGTGGGAA ATGCTTCAAT CAGAGCTCAA 1141 GCCTTATCAT TCACCAGAGA ACCCACACAG GAGAAAAGCC CTATCAATGT GAAGAGTGTG 1201 GAAAAAGCTT CAATAACAGT TCTCATTTTA GTGCACATCG GAGGATACAC ACAGGAGAGA 1261 GACCCCATGT GTGTCCTGAC TGTGGAAAGA GTTTCAGTAA GAGTTCTGAC TTACGTGCAC 1321 ATCATAGAAC CCACACAGGA GAGAAACCCT ATGGGTGTCA TGACTGTGGT AAGTGCTTCA 1381 GTAAAAGCTC TGCCCTTAAT AAGCACGGAG AAATCCATGC ACGGGAAAAG CTTCTGACAC 1441 AGTCAGCTCC CAAGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1501 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGCCCGTAA CTTGAAAGTA 1561 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCGGCCCCAC TTCGGGATTG 1621 CTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt