Construct: ORF TRCN0000466432
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002702.1_s317c1
- Derived from:
- ccsbBroadEn_08844
- DNA Barcode:
- CCGTTCGTGAGCCTCGAGTCACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4A (64218)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466432
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64218 | SEMA4A | semaphorin 4A | NM_001193300.2 | 99.9% | 100% | 1716C>T |
| 2 | human | 64218 | SEMA4A | semaphorin 4A | NM_001193301.1 | 99.9% | 100% | 1716C>T |
| 3 | human | 64218 | SEMA4A | semaphorin 4A | NM_001370567.1 | 99.9% | 100% | 1716C>T |
| 4 | human | 64218 | SEMA4A | semaphorin 4A | NM_022367.4 | 99.9% | 100% | 1716C>T |
| 5 | human | 64218 | SEMA4A | semaphorin 4A | XM_011509872.2 | 99.9% | 100% | 1716C>T |
| 6 | human | 64218 | SEMA4A | semaphorin 4A | XM_011509871.3 | 94.4% | 94.2% | (many diffs) |
| 7 | human | 64218 | SEMA4A | semaphorin 4A | NM_001370568.1 | 86.9% | 86.9% | 0_1ins297;1419C>T |
| 8 | human | 64218 | SEMA4A | semaphorin 4A | XM_011509874.2 | 86.9% | 86.9% | 0_1ins297;1419C>T |
| 9 | human | 64218 | SEMA4A | semaphorin 4A | XM_011509875.3 | 86.9% | 86.9% | 0_1ins297;1419C>T |
| 10 | human | 64218 | SEMA4A | semaphorin 4A | XM_011509878.2 | 86.9% | 86.9% | 0_1ins297;1419C>T |
| 11 | human | 64218 | SEMA4A | semaphorin 4A | NM_001193302.1 | 82.6% | 82.6% | 0_1ins297;66_67ins99;1320C>T |
| 12 | human | 64218 | SEMA4A | semaphorin 4A | NM_001370569.1 | 77.7% | 77.7% | 0_1ins507;1209C>T |
| 13 | human | 64218 | SEMA4A | semaphorin 4A | NM_001370571.1 | 77.7% | 77.7% | 0_1ins507;1209C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2349
- ORF length:
- 2283
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctcccagcc ctgggcctgg acccctggag cctcctgggc cttttcctct 121 tccaactgct tcagctgctg ctgccgacga cgaccgcggg gggaggcggg caggggccca 181 tgcccagggt cagatactat gcaggggatg aacgtagggc acttagcttc ttccaccaga 241 agggcctcca ggattttgac actctgctcc tgagtggtga tggaaatact ctctacgtgg 301 gggctcgaga agccattctg gccttggata tccaggatcc aggggtcccc aggctaaaga 361 acatgatacc gtggccagcc agtgacagaa aaaagagtga atgtgccttt aagaagaaga 421 gcaatgagac acagtgtttc aacttcatcc gtgtcctggt ttcttacaat gtcacccatc 481 tctacacctg cggcaccttc gccttcagcc ctgcttgtac cttcattgaa cttcaagatt 541 cctacctgtt gcccatctcg gaggacaagg tcatggaggg aaaaggccaa agcccctttg 601 accccgctca caagcatacg gctgtcttgg tggatgggat gctctattct ggtactatga 661 acaacttcct gggcagtgag cccatcctga tgcgcacact gggatcccag cctgtcctca 721 agaccgacaa cttcctccgc tggctgcatc atgacgcctc ctttgtggca gccatccctt 781 cgacccaggt cgtctacttc ttcttcgagg agacagccag cgagtttgac ttctttgaga 841 ggctccacac atcgcgggtg gctagagtct gcaagaatga cgtgggcggc gaaaagctgc 901 tgcagaagaa gtggaccacc ttcctgaagg cccagctgct ctgcacccag ccggggcagc 961 tgcccttcaa cgtcatccgc cacgcggtcc tgctccccgc cgattctccc acagctcccc 1021 acatctacgc agtcttcacc tcccagtggc aggttggcgg gaccaggagc tctgcggttt 1081 gtgccttctc tctcttggac attgaacgtg tctttaaggg gaaatacaaa gagttgaaca 1141 aagaaacttc acgctggact acttataggg gccctgagac caacccccgg ccaggcagtt 1201 gctcagtggg cccctcctct gataaggccc tgaccttcat gaaggaccat ttcctgatgg 1261 atgagcaagt ggtggggacg cccctgctgg tgaaatctgg cgtggagtat acacggcttg 1321 cagtggagac agcccagggc cttgatgggc acagccatct tgtcatgtac ctgggaacca 1381 ccacagggtc gctccacaag gctgtggtaa gtggggacag cagtgctcat ctggtggaag 1441 agattcagct gttccctgac cctgaacctg ttcgcaacct gcagctggcc cccacccagg 1501 gtgcagtgtt tgtaggcttc tcaggaggtg tctggagggt gccccgagcc aactgtagtg 1561 tctatgagag ctgtgtggac tgtgtccttg cccgggaccc ccactgtgcc tgggaccctg 1621 agtcccgaac ctgttgcctc ctgtctgccc ccaacctgaa ctcctggaag caggacatgg 1681 agcgggggaa cccagagtgg gcatgtgcca gtggccccat gagcaggagc cttcggcctc 1741 agagccgccc gcaaatcatt aaagaagtcc tggctgtccc taactccatc ctggagctcc 1801 cctgccccca cctgtcagcc ttggcctctt attattggag tcatggccca gcagcagtcc 1861 cagaagcctc ttccactgtc tacaatggct ccctcttgct gatagtgcag gatggagttg 1921 ggggtctcta ccagtgctgg gcaactgaga atggcttttc ataccctgtg atctccTACT 1981 GGGTGGACAG CCAGGACCAG ACCCTGGCCC TGGATCCTGA ACTGGCAGGC ATCCCCCGGG 2041 AGCATGTGAA GGTCCCGTTG ACCAGGGTCA GTGGTGGGGC CGCCCTGGCT GCCCAGCAGT 2101 CCTACTGGCC CCACTTTGTC ACTGTCACTG TCCTCTTTGC CTTAGTGCTT TCAGGAGCCC 2161 TCATCATCCT CGTGGCCTCC CCATTGAGAG CACTCCGGGC TCGGGGCAAG GTTCAGGGCT 2221 GTGAGACCCT GCGCCCTGGG GAGAAGGCCC CGTTAAGCAG AGAGCAACAC CTCCAGTCTC 2281 CCAAGGAATG CAGGACCTCT GCCAGTGATG TGGACGCTGA CAACAACTGC CTAGGCACTG 2341 AGGTAGCTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2401 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2461 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCGTTC GTGAGCCTCG AGTCACGGAC 2521 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt