Construct: ORF TRCN0000466432

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002702.1_s317c1
Derived from:
ccsbBroadEn_08844
DNA Barcode:
CCGTTCGTGAGCCTCGAGTCACGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEMA4A (64218)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466432
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64218 SEMA4A semaphorin 4A NM_001193300.2 99.9% 100% 1716C>T
2 human 64218 SEMA4A semaphorin 4A NM_001193301.1 99.9% 100% 1716C>T
3 human 64218 SEMA4A semaphorin 4A NM_001370567.1 99.9% 100% 1716C>T
4 human 64218 SEMA4A semaphorin 4A NM_022367.4 99.9% 100% 1716C>T
5 human 64218 SEMA4A semaphorin 4A XM_011509872.2 99.9% 100% 1716C>T
6 human 64218 SEMA4A semaphorin 4A XM_011509871.3 94.4% 94.2% (many diffs)
7 human 64218 SEMA4A semaphorin 4A NM_001370568.1 86.9% 86.9% 0_1ins297;1419C>T
8 human 64218 SEMA4A semaphorin 4A XM_011509874.2 86.9% 86.9% 0_1ins297;1419C>T
9 human 64218 SEMA4A semaphorin 4A XM_011509875.3 86.9% 86.9% 0_1ins297;1419C>T
10 human 64218 SEMA4A semaphorin 4A XM_011509878.2 86.9% 86.9% 0_1ins297;1419C>T
11 human 64218 SEMA4A semaphorin 4A NM_001193302.1 82.6% 82.6% 0_1ins297;66_67ins99;1320C>T
12 human 64218 SEMA4A semaphorin 4A NM_001370569.1 77.7% 77.7% 0_1ins507;1209C>T
13 human 64218 SEMA4A semaphorin 4A NM_001370571.1 77.7% 77.7% 0_1ins507;1209C>T
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2349
ORF length:
2283
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cctcccagcc ctgggcctgg acccctggag cctcctgggc cttttcctct
121tccaactgct tcagctgctg ctgccgacga cgaccgcggg gggaggcggg caggggccca
181tgcccagggt cagatactat gcaggggatg aacgtagggc acttagcttc ttccaccaga
241agggcctcca ggattttgac actctgctcc tgagtggtga tggaaatact ctctacgtgg
301gggctcgaga agccattctg gccttggata tccaggatcc aggggtcccc aggctaaaga
361acatgatacc gtggccagcc agtgacagaa aaaagagtga atgtgccttt aagaagaaga
421gcaatgagac acagtgtttc aacttcatcc gtgtcctggt ttcttacaat gtcacccatc
481tctacacctg cggcaccttc gccttcagcc ctgcttgtac cttcattgaa cttcaagatt
541cctacctgtt gcccatctcg gaggacaagg tcatggaggg aaaaggccaa agcccctttg
601accccgctca caagcatacg gctgtcttgg tggatgggat gctctattct ggtactatga
661acaacttcct gggcagtgag cccatcctga tgcgcacact gggatcccag cctgtcctca
721agaccgacaa cttcctccgc tggctgcatc atgacgcctc ctttgtggca gccatccctt
781cgacccaggt cgtctacttc ttcttcgagg agacagccag cgagtttgac ttctttgaga
841ggctccacac atcgcgggtg gctagagtct gcaagaatga cgtgggcggc gaaaagctgc
901tgcagaagaa gtggaccacc ttcctgaagg cccagctgct ctgcacccag ccggggcagc
961tgcccttcaa cgtcatccgc cacgcggtcc tgctccccgc cgattctccc acagctcccc
1021acatctacgc agtcttcacc tcccagtggc aggttggcgg gaccaggagc tctgcggttt
1081gtgccttctc tctcttggac attgaacgtg tctttaaggg gaaatacaaa gagttgaaca
1141aagaaacttc acgctggact acttataggg gccctgagac caacccccgg ccaggcagtt
1201gctcagtggg cccctcctct gataaggccc tgaccttcat gaaggaccat ttcctgatgg
1261atgagcaagt ggtggggacg cccctgctgg tgaaatctgg cgtggagtat acacggcttg
1321cagtggagac agcccagggc cttgatgggc acagccatct tgtcatgtac ctgggaacca
1381ccacagggtc gctccacaag gctgtggtaa gtggggacag cagtgctcat ctggtggaag
1441agattcagct gttccctgac cctgaacctg ttcgcaacct gcagctggcc cccacccagg
1501gtgcagtgtt tgtaggcttc tcaggaggtg tctggagggt gccccgagcc aactgtagtg
1561tctatgagag ctgtgtggac tgtgtccttg cccgggaccc ccactgtgcc tgggaccctg
1621agtcccgaac ctgttgcctc ctgtctgccc ccaacctgaa ctcctggaag caggacatgg
1681agcgggggaa cccagagtgg gcatgtgcca gtggccccat gagcaggagc cttcggcctc
1741agagccgccc gcaaatcatt aaagaagtcc tggctgtccc taactccatc ctggagctcc
1801cctgccccca cctgtcagcc ttggcctctt attattggag tcatggccca gcagcagtcc
1861cagaagcctc ttccactgtc tacaatggct ccctcttgct gatagtgcag gatggagttg
1921ggggtctcta ccagtgctgg gcaactgaga atggcttttc ataccctgtg atctccTACT
1981GGGTGGACAG CCAGGACCAG ACCCTGGCCC TGGATCCTGA ACTGGCAGGC ATCCCCCGGG
2041AGCATGTGAA GGTCCCGTTG ACCAGGGTCA GTGGTGGGGC CGCCCTGGCT GCCCAGCAGT
2101CCTACTGGCC CCACTTTGTC ACTGTCACTG TCCTCTTTGC CTTAGTGCTT TCAGGAGCCC
2161TCATCATCCT CGTGGCCTCC CCATTGAGAG CACTCCGGGC TCGGGGCAAG GTTCAGGGCT
2221GTGAGACCCT GCGCCCTGGG GAGAAGGCCC CGTTAAGCAG AGAGCAACAC CTCCAGTCTC
2281CCAAGGAATG CAGGACCTCT GCCAGTGATG TGGACGCTGA CAACAACTGC CTAGGCACTG
2341AGGTAGCTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
2401CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
2461TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCGTTC GTGAGCCTCG AGTCACGGAC
2521GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)