Construct: ORF TRCN0000466485

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018147.2_s317c1
Derived from:
ccsbBroadEn_04920
DNA Barcode:
TAAAACAGTGGTCAGGCCGCTACT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIP5KL1 (138429)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466485
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 138429 PIP5KL1 phosphatidylinositol-4-phos... NM_173492.1 100% 100%
2 human 138429 PIP5KL1 phosphatidylinositol-4-phos... NM_001135219.2 48.4% 48.4% 1_609del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
639
ORF length:
573
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca gagcgtcttc taccccgccg gccgcatctc cgagaggtat gacatcaaag
121gctgcgaggt gagccgctgg gtggatcccg cccctgaggg cagccccctt gttctggtgc
181tgaaggacct caactttcag ggcaagacca tcaacctggg gccccagcgg agctggttcc
241tccgccagat ggaactggat accaccttcc tccgggagct caacgtgctg gattacagcc
301tcctgatagc cttccaacgt ctccacgagg atgagagggg cccgggcagc agcctcatct
361tccgcacggc caggtctgtg caaggggcac agagcccgga agagtcgaga gcccaaaacc
421gccggctgct gcCCGACGCC CCCAACGCCC TACACATCCT GGACGGGCCC GAGCAGCGCT
481ATTTCCTGGG CGTCGTGGAT CTCGCCACAG TCTACGGGCT CCGCAAGCGG CTGGAGCACC
541TGTGGAAGAC ACTGCGCTAC CCAGGCCGGA CCTTCTCCAC TGTCAGCCCG GCTCGCTACG
601CCCGTCGCCT CTGCCAGTGG GTGGAGGCGC ACACGGAGTG CCCAACTTTC TTGTACAAAG
661TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
721GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
781ACGATAAAAC AGTGGTCAGG CCGCTACTAC GCGTTAAGTC gacaatcaac ctctggatta
841caaaatttgt gaaagatt

Download FASTA (ORF) (Full)