Construct: ORF TRCN0000466486
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000371.1_s317c1
- Derived from:
- ccsbBroadEn_03288
- DNA Barcode:
- ACCGAGTCCGAATAGTTTATTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOOK1 (51361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466486
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51361 | HOOK1 | hook microtubule tethering ... | NM_015888.6 | 100% | 100% | |
| 2 | human | 51361 | HOOK1 | hook microtubule tethering ... | XM_024447520.1 | 94.2% | 94.2% | 0_1ins126 |
| 3 | human | 51361 | HOOK1 | hook microtubule tethering ... | XM_011541562.2 | 93.7% | 93.4% | (many diffs) |
| 4 | human | 51361 | HOOK1 | hook microtubule tethering ... | XR_246271.1 | 86% | 1_196del;1823_1866del;2325_2326ins99 | |
| 5 | human | 51361 | HOOK1 | hook microtubule tethering ... | XM_011541563.1 | 76.7% | 74.6% | 1626_1627ins118;1677_1678ins389 |
| 6 | human | 51361 | HOOK1 | hook microtubule tethering ... | XM_006710676.1 | 75.8% | 74.5% | (many diffs) |
| 7 | human | 51361 | HOOK1 | hook microtubule tethering ... | XM_017001424.1 | 59.3% | 54.5% | (many diffs) |
| 8 | human | 51361 | HOOK1 | hook microtubule tethering ... | XR_946665.1 | 35.6% | 1_196del;1822_1823ins118;2263_5670del | |
| 9 | mouse | 77963 | Hook1 | hook microtubule tethering ... | NM_030014.2 | 87% | 92.5% | (many diffs) |
| 10 | mouse | 77963 | Hook1 | hook microtubule tethering ... | XR_376377.3 | 69.5% | (many diffs) | |
| 11 | mouse | 77963 | Hook1 | hook microtubule tethering ... | XM_006503488.3 | 66.7% | 69% | (many diffs) |
| 12 | mouse | 77963 | Hook1 | hook microtubule tethering ... | XM_006503487.3 | 66.1% | 68.9% | (many diffs) |
| 13 | mouse | 77963 | Hook1 | hook microtubule tethering ... | XR_376374.3 | 49.6% | (many diffs) | |
| 14 | mouse | 77963 | Hook1 | hook microtubule tethering ... | XR_001784220.1 | 31.1% | (many diffs) | |
| 15 | mouse | 77963 | Hook1 | hook microtubule tethering ... | XR_001784219.1 | 27.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2250
- ORF length:
- 2184
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagacgcag ccgccgccgc agcctaagct gcccctgtgc gacagcctca 121 tgatctggct gcagacattc aatactgcct caccttgtca agatgtcaaa cagctgacta 181 gtggagttgc catggcacaa gttcttcatc aaattgatgc agcttggttt aacgaatctt 241 ggttaagccg aattaaagag gatgttgggg acaactggag aataaaggcc agtaatgtaa 301 agaaggtcct tcaaggaatt atgagttatt atcatgagtt tttggggcag cagatttcag 361 aagcacttat ccctgattta aaccaaataa ccgaatgttc agatccagtg gagcttggga 421 ggttgctcca gcttatttta ggttgtgcga tcaactgtga aaagaagcaa gaacatattc 481 aaaatataat gacactggaa gagtctgttc aacatgtggt catgactgct attcaagagt 541 tgatgagtaa agaaatattg agctctcctc caaatgatgc tgttggagaa ttggagcaac 601 agcttaaaag agccttggaa gaacttcagg aagcactagc agaaaaagaa gagctgaggc 661 aaagatgtga agaattggat atgcaggtga ctacacttca agatgaaaag aattcactgg 721 tttctgaaaa tgagatgatg aatgaaaaac ttgaccagtt ggatggctct tttgatgatc 781 caaacacagt ggttgcaaaa aagtattttc atgcacaatt acaactagaa caattacagg 841 aagaaaactt caggcttgaa gctgcaaaag atgattaccg tgttcactgt gaagaacttg 901 aaaagcagct aatcgaattc cagcatagga atgatgaatt gactagtctt gcagaagaaa 961 caagagccct gaaagatgaa atagatgttc ttagggctac ctctgataaa gcaaataaac 1021 tggagtcaac agttgagata tatcgtcaga agctacaaga tctgaatgac cttcgcaagc 1081 aggtgaaaac tttacaggaa accaacatga tgtatatgca taatacagtc agcttagaag 1141 aagaattaaa aaaagcaaat gcagcacgta cacaattaga aacatacaaa aggcaggttc 1201 aagatcttca tgttaaactt tcctccgaat ccaagagggc agacacacta gcgtttgaaa 1261 tgaagcggct tgaagaaaaa catgaagctt tacttaagga aaaagagaga ctaattgagc 1321 agcgtgatac tttgaaagaa acaaatgaag agcttcgatg ttcacaagta caacaggacc 1381 acctaaacca aacagatgca tctgctacaa aaagttatga gaatcttgct gctgagatta 1441 tgccagtgga atatagggag gtgtttattc gactgcaaca tgaaaataag atgcttcgct 1501 tacagcaaga aggctctgag aatgaacgta ttgaggaact tcaggagcag ctagaacaga 1561 aacaccgtaa aatgaatgaa ctggaaactg agcagaggct gagcaaagag cgtattagag 1621 aattgcagca gcagattgag gacctccaga aatctttaca ggaacaaggt tccaagtctg 1681 aaggcgaaag ttccagcaaa ttaaagcaga agttggaagc tcatatggaa aaactcacag 1741 aggtccatga agaattacag aagaaacaag aactcattga agatcttcag ccagatataa 1801 atcaaaatgt acaaaagatc aatgaacttg aagctgctct tcagaagaaa gatgaagata 1861 tgaaagcaat ggaggaaaga tataaaatgt acttggagaa agccagaaat gtaataaaaa 1921 ctttggatCC CAAGTTAAAT CCAGCATCAG CTGAAATAAT GCTACTAAGA AAGCAGTTGG 1981 CAGAGAAAGA GAGAAGAATT GAGATTCTGG AGAGTGAATG CAAAGTAGCA AAATTCCGTG 2041 ATTATGAAGA AAAACTCATT GTTTCTGCGT GGTATAATAA GAGTCTAGCA TTCCAGAAAC 2101 TGGGGATGGA ATCTAGACTT GTGAGCGGCG GTGGTGCCTG CAGTGACACT GGTGCGTGCA 2161 CTCCTGCGCG GTCTTTCTTA GCGCAGCAAC GGCACATCAC CAACACCAGA AGAAATCTCT 2221 CTGTTAAAGT CCCTGCTACA ACATCTGATT ACCCAACTTT CTTGTACAAA GTGGTTGATA 2281 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2341 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACCGA 2401 GTCCGAATAG TTTATTCAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2461 tgaaagatt