Construct: ORF TRCN0000466509
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009498.1_s317c1
- Derived from:
- ccsbBroadEn_07432
- DNA Barcode:
- CAATCCCTTATATCTCACTTAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XAGE2 (9502)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466509
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9502 | XAGE2 | X antigen family member 2 | NM_130777.3 | 99.6% | 100% | 294C>T |
| 2 | human | 9502 | XAGE2 | X antigen family member 2 | XM_011530836.2 | 99.6% | 100% | 294C>T |
| 3 | human | 170626 | XAGE3 | X antigen family member 3 | NM_130776.1 | 86.4% | 72% | (many diffs) |
| 4 | human | 170626 | XAGE3 | X antigen family member 3 | NM_133179.3 | 86.4% | 72% | (many diffs) |
| 5 | human | 170626 | XAGE3 | X antigen family member 3 | XM_011530768.2 | 86.4% | 72% | (many diffs) |
| 6 | human | 170626 | XAGE3 | X antigen family member 3 | XM_011530769.2 | 86.4% | 72% | (many diffs) |
| 7 | human | 170626 | XAGE3 | X antigen family member 3 | XM_011530770.2 | 86.4% | 72% | (many diffs) |
| 8 | human | 170627 | XAGE5 | X antigen family member 5 | NM_130775.2 | 83.8% | 67.5% | (many diffs) |
| 9 | human | 170627 | XAGE5 | X antigen family member 5 | XM_017029320.2 | 83.8% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 399
- ORF length:
- 333
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGAG TTGGCGAGGA AGATCAACAT ATAGGCCTAG GCCAAGAAGA AGTTTACAGC 121 CTCCTGAGCT GATTGGGGCT ATGCTTGAAC CCACTGATGA AGAGCCTAAA GAAGAGAAAC 181 CACCCACTAA AAGTCGGAAT CCTACACCTG ATCAGAAGAG AGAAGATGAT CAGGGTGCAG 241 CTGAGATTCA AGTGCCTGAC CTGGAAGCCG ATCTCCAGGA GCTATGTCAG ACAAAGACTG 301 GGGATGGATG TGAAGGTGGT ACTGATGTCA AGGGGAAGAT TCTACCAAAA GCAGAGCATT 361 TTAAAATGCC AGAAGCAGGT GAAGGGAAAT CACAGGTTTA CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGACAATCC CTTATATCTC ACTTAATCAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt