Construct: ORF TRCN0000466526
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007148.1_s317c1
- Derived from:
- ccsbBroadEn_06787
- DNA Barcode:
- TATTTTCCCCCACATTCGAGTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLK7 (5650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466526
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5650 | KLK7 | kallikrein related peptidase 7 | NM_005046.4 | 99.8% | 100% | 34T>C |
| 2 | human | 5650 | KLK7 | kallikrein related peptidase 7 | NM_139277.2 | 99.8% | 100% | 34T>C |
| 3 | human | 5650 | KLK7 | kallikrein related peptidase 7 | NM_001207053.2 | 71.5% | 71.5% | 0_1ins216 |
| 4 | human | 5650 | KLK7 | kallikrein related peptidase 7 | NM_001243126.1 | 68.8% | 74.4% | 1_127del;161T>C;200_201ins148 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aagatccctt ctcctgcccc tgcagatcct actgctatcc ttagccttgg 121 aaactgcagg agaagaagcc cagggtgaca agattattga tggcgcccca tgtgcaagag 181 gctcccaccc atggcaggtg gccctgctca gtggcaatca gctccactgc ggaggcgtcc 241 tggtcaatga gcgctgggtg ctcactgccg cccactgcaa gatgaatgag tacaccgtgc 301 acctgggcag tgatacgctg ggcgacagga gagctcagag gatcaaggcc tcgaagtcat 361 tccgccaccc cggctactcc acacagaccc atgttaatga ccTCATGCTC GTGAAGCTCA 421 ATAGCCAGGC CAGGCTGTCA TCCATGGTGA AGAAAGTCAG GCTGCCCTCC CGCTGCGAAC 481 CCCCTGGAAC CACCTGTACT GTCTCCGGCT GGGGCACTAC CACGAGCCCA GATGTGACCT 541 TTCCCTCTGA CCTCATGTGC GTGGATGTCA AGCTCATCTC CCCCCAGGAC TGCACGAAGG 601 TTTACAAGGA CTTACTGGAA AATTCCATGC TGTGCGCTGG CATCCCCGAC TCCAAGAAAA 661 ACGCCTGCAA TGGTGACTCA GGGGGACCGT TGGTGTGCAG AGGTACCCTG CAAGGTCTGG 721 TGTCCTGGGG AACTTTCCCT TGCGGCCAAC CCAATGACCC AGGAGTCTAC ACTCAAGTGT 781 GCAAGTTCAC CAAGTGGATA AATGACACCA TGAAAAAGCA TCGCTACCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA TATTTTCCCC CACATTCGAG TGTTACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt