Construct: ORF TRCN0000466527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018513.1_s317c1
- Derived from:
- ccsbBroadEn_00419
- DNA Barcode:
- TCGTTAATGTCTATTGATGGGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAO (1610)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1610 | DAO | D-amino acid oxidase | NM_001917.5 | 100% | 100% | |
| 2 | human | 1610 | DAO | D-amino acid oxidase | XM_011538004.2 | 100% | 100% | |
| 3 | human | 1610 | DAO | D-amino acid oxidase | XM_011538005.2 | 100% | 100% | |
| 4 | human | 1610 | DAO | D-amino acid oxidase | XM_005268692.4 | 93.6% | 93.6% | 385_386ins66 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1107
- ORF length:
- 1041
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg tgtggtggtg attggagcag gagtcatcgg gctgtccacc gccctctgca 121 tccatgagcg ctaccactca gtcctgcagc cactggacat aaaggtctac gcggaccgct 181 tcaccccact caccaccacc gacgtggctg ccggcctctg gcagccctac ctttctgacc 241 ccaacaaccc acaggaggcg gactggagcc aacagacctt tgactatctc ctgagccatg 301 tccattctcc caacgctgaa aacctgggcc tgttcctaat ctcgggctac aacctcttcc 361 atgaagccat tccggaccct tcctggaagg acacagttct gggatttcgg aagctgaccc 421 ccagagagct ggatatgttc ccagattacg gctatggctg gttccacaca agcctaattc 481 tggagggaaa gaactatcta cagtggctga ctgaaaggtt aactgagagg ggagtgaagt 541 tcttccagcg gaaagtggag tcttttgagg aggtggcaag agaaggcgca gacgtgattg 601 tcaactgcac tggggtatgg gctggggcgc tacaacgaga ccccctgctg cagccaggcc 661 gggggcagat catgaaggtg gacgcccctt ggatgaagca cttcattctc acccatgacc 721 cagagagagg catctacaat tccccgtaca tcatcccagg gacccagaca gttactcttg 781 gaggcatctt ccagttggga aactggagtg aactaaacaa tatccaggac cacaacacca 841 tttgGGAAGG CTGCTGCAGA CTGGAGCCCA CACTGAAGAA TGCAAGAATT ATTGGTGAAC 901 GAACTGGCTT CCGGCCAGTA CGCCCCCAGA TTCGGCTAGA AAGAGAACAG CTTCGCACTG 961 GACCTTCAAA CACAGAGGTC ATCCACAACT ATGGCCATGG AGGCTACGGG CTCACCATCC 1021 ACTGGGGATG TGCCCTGGAG GCAGCCAAGC TCTTTGGGAG AATCCTGGAA GAAAAGAAAT 1081 TGTCCAGAAT GCCACCATCC CACCTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCGTTAAT 1261 GTCTATTGAT GGGTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt