Construct: ORF TRCN0000466535
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003370.1_s317c1
- Derived from:
- ccsbBroadEn_08871
- DNA Barcode:
- ATAACTATCCTCTAGGATGTACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IQCH (64799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466535
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64799 | IQCH | IQ motif containing H | NM_001031715.3 | 99.9% | 99.9% | 1956T>C;2794G>C |
| 2 | human | 64799 | IQCH | IQ motif containing H | NM_001322475.2 | 74.2% | 74.1% | (many diffs) |
| 3 | human | 64799 | IQCH | IQ motif containing H | NM_001322470.2 | 70.2% | 70.3% | (many diffs) |
| 4 | human | 64799 | IQCH | IQ motif containing H | NM_001284347.2 | 66.5% | 66.5% | (many diffs) |
| 5 | human | 64799 | IQCH | IQ motif containing H | NM_001322474.2 | 64% | 64% | 0_1ins519;1113_1114ins465;1691_1692ins123 |
| 6 | human | 64799 | IQCH | IQ motif containing H | NM_001322473.2 | 63.7% | 61.1% | (many diffs) |
| 7 | human | 64799 | IQCH | IQ motif containing H | NM_001284348.2 | 60.3% | 57.5% | (many diffs) |
| 8 | human | 64799 | IQCH | IQ motif containing H | NR_147832.2 | 57.1% | (many diffs) | |
| 9 | human | 64799 | IQCH | IQ motif containing H | NM_001322471.2 | 57% | 57% | (many diffs) |
| 10 | human | 64799 | IQCH | IQ motif containing H | NM_001322472.2 | 56.3% | 56.3% | 0_1ins756;876_877ins465;1454_1455ins123 |
| 11 | human | 64799 | IQCH | IQ motif containing H | NM_001284349.1 | 29.1% | 28.3% | (many diffs) |
| 12 | human | 64799 | IQCH | IQ motif containing H | NM_022784.3 | 14.7% | 13% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3147
- ORF length:
- 3081
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc acagaacact gaaaaccacg accctgtcgg atccatctta atccagatcc 121 atgaagacct ttatcagtta aaggagaaat taacaaaatt ctcacctgag gaaaaaggag 181 agactctaga cattcagagt cttgaaacag caatcaaaag gactgaagtg gggttaagaa 241 ttcacattga gaagtattta aatgttgtaa accagaatgt attaacgact tctgttaatg 301 atgagagctt atatactccc caggcttcca aatggttact tccaactgta attgatcaga 361 aatcatttat tttccctcag gaatctgagg gtacattttg gcaaccccaa agacagcaca 421 gttcatctct gcctgtcttt ccaagagcaa agataaaggt ttcgaagtta atcaaagggt 481 ctaacatatc cagcctcacg gttctgccat cttctcattg cacagatccc tatttcactc 541 ctataccagt cttacaagca gatgcccaca aagggatttt aagtatgata gaacgagggc 601 tgattccacc aacagcaagg attacctttc agaatccacc cattacaccc agagcagctc 661 ctctgcatag ttttgatgaa gcacgtaaga ttccaactgt agccactttc actatacctc 721 gggaaccacc tccatctcca gcagaagtga agttctttcc caagaaacaa agatcaaagg 781 ggaaaagcag aaggtcaaga ggacatcatg ataggaaggc catgaaagtc aaaacacctt 841 tgagagccct gaaatcactg tgggattatg actttttaat ttatgatggt gtcatagaca 901 atacagcccc agacttctta gcattcaagg aacattttag cttagcttgg ggaggtattt 961 tttctctctt ggaacacgtc gagaagtttc tcaggaacta tgctatacca gaagtcaaaa 1021 taaaagggaa taatttggtg gccctccttc cagagtttga gctgacgaat aaacttacca 1081 gatatgacct tctctcagtg ttagaggacc cagctcatgt ccaaatgctg ataaatcttc 1141 cagggcaaag gtacaagggc caagatggaa attcggaggc cgccatgaag atccaagcca 1201 catggaaatg ctacaaagca agaaaattct tcctctttta tcgccagcag aagtgggcat 1261 caggtgtgat tgccattgct tggctgttat attgccataa gactcgacta aagaagatac 1321 taaaggaatc acgtcagaga cacctggaga attttcgcat tcgagccaag catctggcag 1381 ccaactggaa tcgcatcagg acctccagga ggactattat ccatatccca tcattagggt 1441 attcccagcc tgtgagagaa catattgccg atttcaacac acagcagaac atgcagctgg 1501 ggaggctgtg tgacatctta gatgccaatg tgaatgtcat ctacatctgc tcccatcata 1561 tgaatgacga gttagtgctg tattacaaaa aaatcctaag tctacatgca gccgtcaaat 1621 ctgggaacct tgaggacaga agtgacctgc aggacaggtt caaaattatc acacctgaag 1681 ctgtaaacat cttccctaag catcatatgt gcctggccac tcacctgatg tacagtccca 1741 aggcaatcaa aagaataaaa aatctcatcc gaggaacaga ggcctacatc gtcagcgggc 1801 tcctccacag agatgattta gctgtggccg atatgttaga catacccatc ctgggctctg 1861 agcctgaact agctcatctt tatagtacca aatctggagg caaacgtgtc tttgacagtg 1921 ccaatgtggc agttcctcct ggaatatatg atatttatag tcagcaacag atgatagagc 1981 agctgagtca gctgataact gatcacctgc aaatacagcg ctggctcttt aaaatggact 2041 ctgagttccg aggaaatggg actgcatttt gtgatattcc ttcctaccta aagtgctaca 2101 aatgggtgct aaaggagagt agcagatatg gccttgaaga ctggagaaag aaatgggcac 2161 aagagccagc tttggtgaag atctctgagg agctggcggg cattttagca cagcacgcac 2221 agccagtcaa tgagaaacgg ttcccgacgt ggaggaaatt cctccaaaca tttctcagtc 2281 aagggggtgt gatcgaagca ttcccacctg cagacaatgt caccaacctc acagtggaca 2341 tgctgataga gcccaacggg aaaatcagcg tgctgtcgac aggggaccag cttcatgctg 2401 aaagcccctt catctcctct ggtaccaccg tgcctcagac ctcagtggat ccccaagttc 2461 tcacttattt gtgcctccaa attggaaaag cctgcagaat gagagatgtg gttggttact 2521 tttcgataga tctggtgact tttatagatc caagcacctt ggaacaacag gtgtgggcaa 2581 ccggccttaa cctcgcatat agtgaccagc tggccctgac tcaactcacc ttatacctga 2641 caaacggcca tctggattgc agtttgagca ccctggaagt gccccgcttt gttccaaagg 2701 aaaggaagaa aaccaaatgc atgagtgcgc tgtcaatgcc gatgctggca accagtcgct 2761 atgcagtgat gaccacccag ctaagacaca gcaatctctc actggttttc cactatgttt 2821 ttctccagat ctgtagggcc catggcattg gctatgatct tgaggaaagg caaggaactg 2881 tttttatatt atatgagcac ctgaagagac acaagttggg aatgttaaca atcggcgagg 2941 atctccaggg ggtcctcatg acctttgctc gccatctctt catcatccat caagaaatat 3001 cagcaccTAA TATGCAAGGC GAGACCAATT TTAAGACCAC CATTGCTGAT ATTGAAACTA 3061 TTCTAAGAGT AACAAAGGAA AACAAAATGA GATTTGAAGA GGAGCAACAG TCCAAAGATG 3121 ATAAAAACCT CTCTAAACCC AAGAAATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 3181 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3241 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATAACTAT 3301 CCTCTAGGAT GTACCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3361 aagatt