Transcript: Human NM_001322472.2

Homo sapiens IQ motif containing H (IQCH), transcript variant 8, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
IQCH (64799)
Length:
3202
CDS:
405..2144

Additional Resources:

NCBI RefSeq record:
NM_001322472.2
NBCI Gene record:
IQCH (64799)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001322472.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000127659 CCCAGACTTCTTAGCATTCAA pLKO.1 491 CDS 100% 5.625 4.500 N IQCH n/a
2 TRCN0000127534 CCTGACTCAACTCACCTTATA pLKO.1 1733 CDS 100% 13.200 9.240 N IQCH n/a
3 TRCN0000251889 GGACCTCCAGGAGGACTATTA pLKO_005 982 CDS 100% 13.200 9.240 N Iqch n/a
4 TRCN0000129788 CCAAGAAATGATCCTGGAATA pLKO.1 2134 CDS 100% 10.800 7.560 N IQCH n/a
5 TRCN0000130936 CCCGCTTTGTTCCAAAGGAAA pLKO.1 1801 CDS 100% 4.950 3.465 N IQCH n/a
6 TRCN0000129589 CCTCCGCATTTACTTCCTCAT pLKO.1 2446 3UTR 100% 4.050 2.835 N IQCH n/a
7 TRCN0000129439 GCATCAGGTGTGATTGCCATT pLKO.1 840 CDS 100% 4.050 2.835 N IQCH n/a
8 TRCN0000130790 GCTGACTCTAACTTTCAGCAT pLKO.1 2940 3UTR 100% 0.264 0.185 N IQCH n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001322472.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08871 pDONR223 100% 56.3% 56.3% None 0_1ins756;876_877ins465;1454_1455ins123 n/a
2 ccsbBroad304_08871 pLX_304 0% 56.3% 56.3% V5 0_1ins756;876_877ins465;1454_1455ins123 n/a
3 TRCN0000466535 ATAACTATCCTCTAGGATGTACCC pLX_317 4.8% 56.3% 56.3% V5 0_1ins756;876_877ins465;1454_1455ins123 n/a
4 ccsbBroadEn_15974 pDONR223 0% 38% 36.6% None (many diffs) n/a
5 ccsbBroad304_15974 pLX_304 0% 38% 36.6% V5 (many diffs) n/a
6 TRCN0000469359 CAACTGTCTGTAAAGCGAATCGCG pLX_317 60.3% 38% 36.6% V5 (many diffs) n/a
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