Construct: ORF TRCN0000466572
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018216.1_s317c1
- Derived from:
- ccsbBroadEn_10849
- DNA Barcode:
- TTTACATGGTTTTCAGCCCCTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GMFB (2764)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466572
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2764 | GMFB | glia maturation factor beta | NM_004124.3 | 91.7% | 91.5% | 418delT;426_427ins37 |
| 2 | mouse | 63985 | Gmfb | glia maturation factor, beta | NM_022023.2 | 83.5% | 89.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGAG TGAGTCTTTG GTTGTTTGTG ATGTTGCCGA AGATTTAGTG GAAAAGCTGA 121 GAAAGTTTCG TTTTCGCAAA GAAACGAACA ACGCTGCTAT TATAATGAAG ATTGACAAGG 181 ATAAACGCCT GGTGGTACTG GATGAGGAGC TTGAGGGCAT TTCACCAGAT GAACTTAAAG 241 ATGAACTACC TGAACGACAA CCTCGCTTCA TTGTGTATAG TTATAAATAT CAACATGATG 301 ATGGAAGAGT TTCATATCCT CTGTGCTTTA TTTTCTCCAG TCCTGTTGGA TGTAAGCCTG 361 AACAACAGAT GATGTATGCT GGAAGTAAGA ATAAGCTAGT CCAGACAGCT GAACTAACCA 421 AGGTATTTGA AATAAGAAAT ACCGAAGACC TAACTGAAGA ATGGTTACGT GAGAAACTTG 481 GATTTTTCAC TAATGTGAAC TTCTGTGTTT CTAAAGTATT TATGTATTAC CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGATTTACAT GGTTTTCAGC CCCTTGAACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt