Construct: ORF TRCN0000466582
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012740.1_s317c1
- Derived from:
- ccsbBroadEn_01380
- DNA Barcode:
- TTGCTGGAGAGACTACCACGTTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBBP7 (5931)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466582
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5931 | RBBP7 | RB binding protein 7, chrom... | NM_002893.4 | 100% | 100% | |
| 2 | human | 5931 | RBBP7 | RB binding protein 7, chrom... | NM_001198719.2 | 89.9% | 89.3% | (many diffs) |
| 3 | mouse | 245688 | Rbbp7 | retinoblastoma binding prot... | NM_009031.3 | 93.6% | 99.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1344
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgagtaaa gagatgtttg aagatactgt ggaggagcgt gtcatcaatg 121 aagaatataa aatctggaag aagaatacac cgtttctata tgacctggtt atgacccatg 181 ctcttcagtg gcccagtctt accgttcagt ggcttcctga agtgactaaa cctgaaggaa 241 aagattatgc ccttcattgg ctagtgctgg ggactcatac gtctgatgag cagaatcatc 301 tggtggttgc tcgagtacat attcccaatg atgatgcaca gtttgatgct tcccattgtg 361 acagtgacaa gggtgaattt ggtggctttg gttctgtaac aggaaaaatt gaatgtgaaa 421 ttaaaatcaa tcacgaagga gaagtaaacc gtgctcgtta catgccgcag aatcctcaca 481 tcattgctac aaaaacacca tcttctgatg tgttggtttt tgactataca aaacaccctg 541 ctaaaccaga cccaagtgga gaatgtaatc ctgatctcag attaagaggt caccagaagg 601 aaggctatgg tctctcctgg aattcaaatt tgagtggaca tctcctaagt gcatctgatg 661 accatactgt ttgtctgtgg gatataaacg caggaccaaa agaaggcaaa attgtggatg 721 ctaaagccat ctttactggc cactcagctg ttgtagagga tgtggcctgg cacctgctgc 781 acgagtcatt gtttggatct gttgctgatg atcagaaact tatgatatgg gacaccaggt 841 ccaataccac ctccaagccg agtcacttgg tggatgcgca cactgccgaa gtcaactgcc 901 tctcattcaa tcccTACAGC GAATTTATTC TAGCCACCGG CTCTGCGGAT AAGACCGTAG 961 CTTTATGGGA TCTGCGTAAC TTAAAATTAA AACTCCATAC CTTCGAATCT CATAAAGATG 1021 AAATTTTCCA GGTCCACTGG TCTCCACATA ATGAAACTAT TCTGGCTTCA AGTGGTACTG 1081 ACCGCCGCCT GAATGTGTGG GATTTAAGTA AAATTGGGGA AGAACAATCA GCAGAAGATG 1141 CAGAAGATGG GCCTCCAGAA CTCCTGTTTA TTCATGGAGG ACACACTGCT AAGATTTCAG 1201 ATTTTAGCTG GAACCCCAAT GAGCCTTGGG TCATTTGCTC AGTGTCTGAG GATAACATCA 1261 TGCAGATATG GCAAATGGCT GAAAATATTT ACAATGATGA AGAGTCAGAT GTCACGACAT 1321 CCGAACTGGA GGGACAAGGA TCTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1381 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1441 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TGCTGGAGAG 1501 ACTACCACGT TGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1561 att