Construct: ORF TRCN0000466610
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014572.1_s317c1
- Derived from:
- ccsbBroadEn_07841
- DNA Barcode:
- GGATCTTAATCTCCTCTTCAGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRCH1 (23143)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466610
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23143 | LRCH1 | leucine rich repeats and ca... | NM_001164211.2 | 99.8% | 99.8% | 282C>T;700T>C;2061A>G |
| 2 | human | 23143 | LRCH1 | leucine rich repeats and ca... | NM_015116.3 | 95.2% | 95.2% | (many diffs) |
| 3 | human | 23143 | LRCH1 | leucine rich repeats and ca... | XM_017020483.2 | 94.1% | 92.2% | (many diffs) |
| 4 | human | 23143 | LRCH1 | leucine rich repeats and ca... | NM_001164213.2 | 89.5% | 87.6% | (many diffs) |
| 5 | human | 23143 | LRCH1 | leucine rich repeats and ca... | XM_017020484.1 | 61% | 59.1% | (many diffs) |
| 6 | human | 23143 | LRCH1 | leucine rich repeats and ca... | XR_001749525.2 | 45.2% | (many diffs) | |
| 7 | mouse | 380916 | Lrch1 | leucine-rich repeats and ca... | XM_006519155.3 | 84.7% | 86.4% | (many diffs) |
| 8 | mouse | 380916 | Lrch1 | leucine-rich repeats and ca... | XM_006519154.3 | 81.8% | 83.4% | (many diffs) |
| 9 | mouse | 380916 | Lrch1 | leucine-rich repeats and ca... | XM_006519157.3 | 81.7% | 81.6% | (many diffs) |
| 10 | mouse | 380916 | Lrch1 | leucine-rich repeats and ca... | XM_006519158.3 | 80.3% | 82% | (many diffs) |
| 11 | mouse | 380916 | Lrch1 | leucine-rich repeats and ca... | XM_017316067.1 | 79.2% | 80.1% | (many diffs) |
| 12 | mouse | 380916 | Lrch1 | leucine-rich repeats and ca... | XM_006519156.2 | 76.5% | 77.4% | (many diffs) |
| 13 | mouse | 380916 | Lrch1 | leucine-rich repeats and ca... | XM_006519160.3 | 61.9% | 62.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2358
- ORF length:
- 2289
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgacgccg ggaagcgaac cccaaccttt cgtcccggcc ctttcggtag 121 ctactctgca cccacttcat catccccacc accaccacca ccaccatcag caccacggag 181 gaaccggcgc ccccggcggg gcgggtggtg gcggcggtgg cagcgggggc ttcaacctgc 241 ccttgaaccg gggtctggag cgcgcgcttg aggaggcggc caactccggg gggctgaacc 301 tgagcgccag gaaattgaag gaatttcccc gtaccgcagc ccccgggcat gacctctcgg 361 acacggtgca ggcagactta tctaaaaaca gactggttga agttccaatg gaattgtgcc 421 attttgtatc actggaaatt cttaatctgt atcacaactg tatcagagtc attcctgagg 481 ccatcgttaa tctgcagatg ctgacttacc tgaacttgag tcgaaatcag ctgtccgccc 541 tgcctgcctg cctgtgtggt ctgcctctca aagtcttaat cgcaagtaac aacaaacttg 601 gatcattacc agaagagata ggtcagctca aacagttaat ggagctggat gtcagctgca 661 acgagatcac agcgttgccc cagcagatag gtcagttgaa atctctacga gaactgaatg 721 tcagaagaaa ttaccttaaa gttttaccac aagaactagt agatcttccc ttggtaaagt 781 ttgacttttc ctgcaacaaa gtgctcgtga ttccaatttg ttttagagag atgaagcagc 841 tgcaagtgtt actacttgag aataaccctc tgcagtctcc tccagcacag atttgcacaa 901 agggcaaagt tcacatattt aagtatctga gcatacaagc atgccagatt aagacagctg 961 actcccttta tctccacacc atggagaggc cacatttaca ccagcacgtg gaagatggca 1021 agaaggattc tgattcggga gttggaagtg ataatggaga taagcgatta tctgccaccg 1081 agccttctga cgaagacact gttagcctca atgtgccaat gtcaaacatc atggaagaag 1141 aacagatcat caaggaggac tcgtgccatc gccttagccc cgttaaaggg gaatttcatc 1201 aggaatttca accggagcct tcccttttgg gtgacagcac caactcagga gaagaaagag 1261 accagtttac tgatagagca gatggtctcc attcggaatt tatgaactat aaggcaaggg 1321 cagaagactg tgaagagctg ttacggatag aagaggatgt gcactggcaa actgagggca 1381 taataagttc atccaaagat caggacatgg atatagcaat gatcgagcag ctgagagaag 1441 cagtagattt gctgcaagat cccaatggat taagcacaga tattacagag agaagtgttt 1501 taaacctata tcctatggga tcagcagaag ccttagaatt acaagattct gcactgaatg 1561 gtcaaataca gctggagaca tctccggtgt gtgaggtgca aagtgatcta acattacaga 1621 gtaacgggag ccagtattct ccaaatgaga ttagagagaa ctcccctgca gtctctccta 1681 ccacaaacag cacagctcca tttggcctga agcctcgatc agacccagcc ctcattcttc 1741 ctcctatctc cttcaacaca cttacacagg cacagacatg ggacagctct agctacagtg 1801 ttccatctga aggagacagt gacaatgtgt ttctaagacc tcagagaaat ttggaatcta 1861 tagacccgca gtttacaatc cggaggaaaa tggagcagat gagagaagag aaagagctgg 1921 tggaacaact tcgtgagagc attgagatga gattgaaggt cagtctacac gaagacctgg 1981 gggcagccct catggatggt gtcgtccTCT GCCATCTGGT CAACCACATC CGCCCACGGT 2041 CGGTTGCAAG CATCCATGTC CCATCACCAG CGGTTCCCAA ACTTAGCATG GCCAAATGCA 2101 GAAGAAATGT GGAAAACTTT TTGGAAGCGT GCCGAAAATT AGGAGTACCA GAGGCTGACC 2161 TCTGCTCTCC GTGTGACATC CTGCAGTTGG ATTTTCGTCA CATTCGAAAG ACTGTTGACA 2221 CTCTGCTGGC ACTCGGGGAG AAAGCCCCAC CACCAACTTC TGCCCTCCGC TCCAGGGACC 2281 TTATAGGCTT CTGTCTTGTC CATATTCTCT TTATAGTGCT GGTCTATATC ACTTACCACT 2341 GGAATGCTCT GTCCGCATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2401 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2461 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGATCTT AATCTCCTCT 2521 TCAGGCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt