Construct: ORF TRCN0000466634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013574.1_s317c1
- Derived from:
- ccsbBroadEn_11916
- DNA Barcode:
- TTTAGGCCAATCCAAGCGCCTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- A1CF (29974)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29974 | A1CF | APOBEC1 complementation factor | NM_001198818.1 | 20.7% | 20.8% | 366_1758delinsG |
| 2 | human | 29974 | A1CF | APOBEC1 complementation factor | NM_001370130.1 | 20.7% | 20.8% | 366_1758delinsG |
| 3 | human | 29974 | A1CF | APOBEC1 complementation factor | NM_014576.4 | 20.7% | 20.8% | 366_1758delinsG |
| 4 | human | 29974 | A1CF | APOBEC1 complementation factor | NM_001370131.1 | 20.4% | 20.5% | 366_1782delinsG |
| 5 | human | 29974 | A1CF | APOBEC1 complementation factor | NM_138932.2 | 20.4% | 20.5% | 366_1782delinsG |
| 6 | human | 29974 | A1CF | APOBEC1 complementation factor | XM_005269718.2 | 20.4% | 20.5% | 366_1782delinsG |
| 7 | human | 29974 | A1CF | APOBEC1 complementation factor | XM_011539730.2 | 19.6% | 17.4% | (many diffs) |
| 8 | human | 29974 | A1CF | APOBEC1 complementation factor | NM_001198820.1 | 18% | 16.1% | (many diffs) |
| 9 | human | 29974 | A1CF | APOBEC1 complementation factor | NM_138933.2 | 18% | 16.1% | (many diffs) |
| 10 | human | 29974 | A1CF | APOBEC1 complementation factor | NM_001198819.1 | 17.8% | 15.8% | (many diffs) |
| 11 | human | 29974 | A1CF | APOBEC1 complementation factor | XM_011539729.3 | 17.8% | 15.8% | (many diffs) |
| 12 | mouse | 69865 | A1cf | APOBEC1 complementation factor | XM_006527331.1 | 19.1% | 20.1% | (many diffs) |
| 13 | mouse | 69865 | A1cf | APOBEC1 complementation factor | NM_001081074.1 | 18.8% | 19.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 432
- ORF length:
- 366
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGGA ATCAAATCAC AAATCCGGGG ATGGATTGAG CGGCACTCAG AAGGAAGCAG 121 CCCTCCGCGC ACTGGTCCAG CGCACAGGAT ATAGCTTGGT CCAGGAAAAT GGACAAAGAA 181 AATATGGTGG CCCTCCACCT GGTTGGGATG CTGCACCCCC TGAAAGGGGC TGTGAAATTT 241 TTATTGGAAA ACTTCCCCGA GACCTTTTTG AGGATGAGCT TATACCATTA TGTGAAAAAA 301 TCGGTAAAAT TTATGAAATG AGAATGATGA TGGATTTTAA TGGCAACAAT AGAGGATATG 361 CATTTGTAAC ATTTTCAAAT AAAGTGGAAG CCAAGAATGC AATCAAGCAA CTTAATAATT 421 ATGAAATTAG GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 481 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 541 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT AGGCCAATCC AAGCGCCTCC 601 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t