Construct: ORF TRCN0000466653
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013367.1_s317c1
- Derived from:
- ccsbBroadEn_09346
- DNA Barcode:
- TCTGACGTACATCTACAACACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DTD1 (92675)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466653
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92675 | DTD1 | D-aminoacyl-tRNA deacylase 1 | NM_080820.6 | 99.8% | 99.5% | 280C>A |
| 2 | human | 92675 | DTD1 | D-aminoacyl-tRNA deacylase 1 | XM_005260872.4 | 78.7% | 78.4% | 0_1ins132;148C>A |
| 3 | human | 92675 | DTD1 | D-aminoacyl-tRNA deacylase 1 | NM_001318043.2 | 78.5% | 67.9% | (many diffs) |
| 4 | mouse | 66044 | Dtd1 | D-tyrosyl-tRNA deacylase 1 | NM_025314.3 | 88.1% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggccgtggtg cagcgcgtca cccgggccag cgtcacagtt ggaggagagc 121 agattagtgc cattggaagg ggcatatgtg tgttgctggg tatttccctg gaggatacgc 181 agaaggaact ggaacacatg gtccgaaaga ttctaaacct gcgtgtattt gaggatgaga 241 gtgggaagca ctgGTCGAAG AGTGTGATGG ACAAACAGTA CGAGATTCTG TGTGTCAGCC 301 AGTTTACCCT CCAGTGTGTC CTGAAGGGAA ACAAGCCTGA TTTCAACCTA GCAATGCCCA 361 CGGAGCAGGC AGAGGGCTTC TACAACAGCT TCCTGGAGCA GCTGCGTAAA ACATACAGGC 421 CGGAGCTTAT CAAAGATGGC AAGTTTGGGG CCTACATGCA GGTGCACATT CAGAATGATG 481 GGCCTGTGAC CATAGAGCTG GAATCGCCAG CTCCCGGCAC TGCTACCTCT GACCCAAAGC 541 AGCTGTCAAA GCTCGAAAAA CAGCAGCAGA GGAAAGAAAA GACCAGAGCT AAGGGACCTT 601 CTGAATCAAG CAAGGAAAGA AACACTCCCC GAAAAGAAGA CCGCAGTGCC AGCAGCGGGG 661 CTGAGGGCGA CGTGTCCTCT GAACGGGAGC CGTACCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 841 TGACGTACAT CTACAACACT ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt