Construct: ORF TRCN0000466664
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009043.1_s317c1
- Derived from:
- ccsbBroadEn_12519
- DNA Barcode:
- ACTATCCCACAGTAGTGTCCTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHACTR4 (65979)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466664
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001350161.2 | 89% | 85.2% | (many diffs) |
| 2 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001048183.3 | 87% | 83.3% | (many diffs) |
| 3 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001350160.2 | 87% | 83.3% | (many diffs) |
| 4 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001350158.2 | 86% | 82.4% | (many diffs) |
| 5 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_001350159.2 | 86% | 82.4% | (many diffs) |
| 6 | human | 65979 | PHACTR4 | phosphatase and actin regul... | NM_023923.4 | 85.8% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1956
- ORF length:
- 1890
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cctggacagt gtggaagcag gagacacaac acctcctacc aaaaggaaga 121 gcaagttctc aggctttggc aagatcttca agccctggaa atggaggaaa aaaaaaagta 181 gtgataaatt taaagagact tcagaagttt tagaacggaa aatatctatg cgaaagccaa 241 gagaagagct ggttaagaga ggggttctgt tggaagaccc tgagcaaggt ggtgaggatc 301 caggaaagcc aagcgatgcc atgttaaaga atggccatac cacccccata gggaatgcca 361 gatcatctag tccagtccaa gtagaggaag agccagtaag attagcaagt cttaggaaag 421 ctattccaga agaggaccta aagaaacgac taggctcaac tggaagccag cctaattctg 481 aagcagagtc tgttcctgag aatgtaccca aaccaccttt acttcctccc aaaagaccct 541 tgtcctcttc tcatgaagca agtgaagggc aagcaaagga tgccacttcc tctggcggca 601 cggcaaggtt catcatctcc acctccatca ccacagcacc cgctgccacc actgctgcca 661 caagccttgc aaagactgtt aatctctctg tcaccccttc cccagcaccc aggactctgc 721 ctgctgctcc tgccagcact aacactactg ctaccccaag cctcactcat atggtccctg 781 ccaagcagcc ccctatccct ccccctaaac cagctcacag aaatagcaac cctgtcattg 841 ctgaactgtc ccaagcaata aacagtggta cattgttatc aaaaccgtcc ccacccttac 901 cacctaagag aggcattcca tcaacctcag tacccacctt ggagtctgct gctgccatca 961 ccacaaaaac accaagtgat gaaagagaga agagcacgtg ttctatgggc tcggaactac 1021 taccaatgat ctcacctcgc tctccgtccc ccccactgcc tactcatata cctccagagc 1081 ctccacgcac ccctccattc cctgctaaga cttttcaagt tgtgccagaa attgagtttc 1141 caccatcctt agatctacac caggagattc cccagcagga agatcagaaa aaggaagtcc 1201 ccaagaggat actggaccag aactttgggg agccccatat accctctagg ctgcctccac 1261 tcccactgca tattcgaatc cagcaggccc tcaccagccc acttcccatg actcctattc 1321 tggagggttc tcacagagct cattcgttgc tttttgaaaa cagtgacagc ttttctgagg 1381 acagcagtac gctgggtcgg accaggtctc ttcccatcac tattgaaatg ctaaaagttc 1441 cagacgatga agaagaagag gagcaaacct gtccatccac attcagtgaa gaaatgacac 1501 ctacctcagt cattcctaaa ttaccacagt gtctacggga ggaagaagag aaggagagcg 1561 actcTGATTC AGAAGGTCCC ATTCAGTACC GAGATGAAGA AGATGAAGAT GAAAGCTATC 1621 AGAGTGCTCT CGCCAACAAA GTGAAGAGGA AAGACACACT GGCAATGAAG TTGAACCACA 1681 GACCCAGTGA ACCAGAGTTG AACCTGAATT CTTGGCCTTG TAAAAGCAAG GAGGAGTGGA 1741 ATGAAATACG GCACCAGATT GGAAACACAC TGATCCGGCG ACTGAGTCAA AGACCAACAC 1801 CAGAAGAACT AGAACAACGC AATATATTGC AACAATATAG AGTGAAATTT GGCCTTAAAA 1861 GTCTCCTCAC TGAGAAGGTT TGGGCTTCCT TGGGGCTATT TGTGAGCATG TTCAGTCTGA 1921 CCAATCCATC CCCTGTTCTC TCAGCACTAC TTGGCTACCC AACTTTCTTG TACAAAGTGG 1981 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2041 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2101 AACTATCCCA CAGTAGTGTC CTTCGACGCG TTAAGTCgac aatcaacctc tggattacaa 2161 aatttgtgaa agatt