Construct: ORF TRCN0000466664

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009043.1_s317c1
Derived from:
ccsbBroadEn_12519
DNA Barcode:
ACTATCCCACAGTAGTGTCCTTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHACTR4 (65979)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466664
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 65979 PHACTR4 phosphatase and actin regul... NM_001350161.2 89% 85.2% (many diffs)
2 human 65979 PHACTR4 phosphatase and actin regul... NM_001048183.3 87% 83.3% (many diffs)
3 human 65979 PHACTR4 phosphatase and actin regul... NM_001350160.2 87% 83.3% (many diffs)
4 human 65979 PHACTR4 phosphatase and actin regul... NM_001350158.2 86% 82.4% (many diffs)
5 human 65979 PHACTR4 phosphatase and actin regul... NM_001350159.2 86% 82.4% (many diffs)
6 human 65979 PHACTR4 phosphatase and actin regul... NM_023923.4 85.8% 82.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1956
ORF length:
1890
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt cctggacagt gtggaagcag gagacacaac acctcctacc aaaaggaaga
121gcaagttctc aggctttggc aagatcttca agccctggaa atggaggaaa aaaaaaagta
181gtgataaatt taaagagact tcagaagttt tagaacggaa aatatctatg cgaaagccaa
241gagaagagct ggttaagaga ggggttctgt tggaagaccc tgagcaaggt ggtgaggatc
301caggaaagcc aagcgatgcc atgttaaaga atggccatac cacccccata gggaatgcca
361gatcatctag tccagtccaa gtagaggaag agccagtaag attagcaagt cttaggaaag
421ctattccaga agaggaccta aagaaacgac taggctcaac tggaagccag cctaattctg
481aagcagagtc tgttcctgag aatgtaccca aaccaccttt acttcctccc aaaagaccct
541tgtcctcttc tcatgaagca agtgaagggc aagcaaagga tgccacttcc tctggcggca
601cggcaaggtt catcatctcc acctccatca ccacagcacc cgctgccacc actgctgcca
661caagccttgc aaagactgtt aatctctctg tcaccccttc cccagcaccc aggactctgc
721ctgctgctcc tgccagcact aacactactg ctaccccaag cctcactcat atggtccctg
781ccaagcagcc ccctatccct ccccctaaac cagctcacag aaatagcaac cctgtcattg
841ctgaactgtc ccaagcaata aacagtggta cattgttatc aaaaccgtcc ccacccttac
901cacctaagag aggcattcca tcaacctcag tacccacctt ggagtctgct gctgccatca
961ccacaaaaac accaagtgat gaaagagaga agagcacgtg ttctatgggc tcggaactac
1021taccaatgat ctcacctcgc tctccgtccc ccccactgcc tactcatata cctccagagc
1081ctccacgcac ccctccattc cctgctaaga cttttcaagt tgtgccagaa attgagtttc
1141caccatcctt agatctacac caggagattc cccagcagga agatcagaaa aaggaagtcc
1201ccaagaggat actggaccag aactttgggg agccccatat accctctagg ctgcctccac
1261tcccactgca tattcgaatc cagcaggccc tcaccagccc acttcccatg actcctattc
1321tggagggttc tcacagagct cattcgttgc tttttgaaaa cagtgacagc ttttctgagg
1381acagcagtac gctgggtcgg accaggtctc ttcccatcac tattgaaatg ctaaaagttc
1441cagacgatga agaagaagag gagcaaacct gtccatccac attcagtgaa gaaatgacac
1501ctacctcagt cattcctaaa ttaccacagt gtctacggga ggaagaagag aaggagagcg
1561actcTGATTC AGAAGGTCCC ATTCAGTACC GAGATGAAGA AGATGAAGAT GAAAGCTATC
1621AGAGTGCTCT CGCCAACAAA GTGAAGAGGA AAGACACACT GGCAATGAAG TTGAACCACA
1681GACCCAGTGA ACCAGAGTTG AACCTGAATT CTTGGCCTTG TAAAAGCAAG GAGGAGTGGA
1741ATGAAATACG GCACCAGATT GGAAACACAC TGATCCGGCG ACTGAGTCAA AGACCAACAC
1801CAGAAGAACT AGAACAACGC AATATATTGC AACAATATAG AGTGAAATTT GGCCTTAAAA
1861GTCTCCTCAC TGAGAAGGTT TGGGCTTCCT TGGGGCTATT TGTGAGCATG TTCAGTCTGA
1921CCAATCCATC CCCTGTTCTC TCAGCACTAC TTGGCTACCC AACTTTCTTG TACAAAGTGG
1981TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2041CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2101AACTATCCCA CAGTAGTGTC CTTCGACGCG TTAAGTCgac aatcaacctc tggattacaa
2161aatttgtgaa agatt

Download FASTA (ORF) (Full)