Transcript: Human NM_023923.4

Homo sapiens phosphatase and actin regulator 4 (PHACTR4), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
PHACTR4 (65979)
Length:
6189
CDS:
236..2374

Additional Resources:

NCBI RefSeq record:
NM_023923.4
NBCI Gene record:
PHACTR4 (65979)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_023923.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358953 GAACTAGAACAACGCAATATA pLKO_005 2054 CDS 100% 15.000 21.000 N PHACTR4 n/a
2 TRCN0000052819 CGCAATATATTGCAACCTAAA pLKO.1 2066 CDS 100% 10.800 15.120 N PHACTR4 n/a
3 TRCN0000052821 CCCATTCAGTACCGAGATGAA pLKO.1 1826 CDS 100% 4.950 6.930 N PHACTR4 n/a
4 TRCN0000377396 CAAATGTGTAGGCTCTAAATT pLKO_005 2765 3UTR 100% 15.000 10.500 N PHACTR4 n/a
5 TRCN0000369982 TCATGAAGAGAGCAAACATTT pLKO_005 2332 CDS 100% 13.200 9.240 N PHACTR4 n/a
6 TRCN0000358952 ATTCCAGAAGAGGACCTAAAG pLKO_005 671 CDS 100% 10.800 7.560 N PHACTR4 n/a
7 TRCN0000216930 CACTAGAAAGCTCAGTCAAAG pLKO.1 2134 CDS 100% 10.800 7.560 N Phactr4 n/a
8 TRCN0000358950 CACTTCCCATGACTCCTATTC pLKO_005 1548 CDS 100% 10.800 7.560 N PHACTR4 n/a
9 TRCN0000358951 GCAATAAACAGTGGTACATTG pLKO_005 1103 CDS 100% 10.800 7.560 N PHACTR4 n/a
10 TRCN0000052820 CCATAGGGAATGCCAGATCAT pLKO.1 594 CDS 100% 4.950 3.465 N PHACTR4 n/a
11 TRCN0000052818 CCCATCACTATTGAAATGCTA pLKO.1 1661 CDS 100% 3.000 2.100 N PHACTR4 n/a
12 TRCN0000052822 GCCATGTTAAAGAATGGCCAT pLKO.1 566 CDS 100% 0.216 0.151 N PHACTR4 n/a
13 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 3555 3UTR 100% 4.950 2.475 Y GJD4 n/a
14 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 3555 3UTR 100% 4.950 2.475 Y C9orf85 n/a
15 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3115 3UTR 100% 4.950 2.475 Y ERAP2 n/a
16 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3116 3UTR 100% 13.200 6.600 Y LIAS n/a
17 TRCN0000162166 CAACATGATGAAACCCTGTAT pLKO.1 4705 3UTR 100% 4.950 2.475 Y SWSAP1 n/a
18 TRCN0000164260 CCAACATGATGAAACCCTGTA pLKO.1 4704 3UTR 100% 4.050 2.025 Y SWSAP1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_023923.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08894 pDONR223 100% 98.4% 98% None (many diffs) n/a
2 ccsbBroad304_08894 pLX_304 0% 98.4% 98% V5 (many diffs) n/a
3 TRCN0000477735 TCGTTATACATTGCGCACCCGGGC pLX_317 19% 98.4% 98% V5 (many diffs) n/a
4 ccsbBroadEn_12519 pDONR223 100% 85.8% 82.2% None (many diffs) n/a
5 ccsbBroad304_12519 pLX_304 0% 85.8% 82.2% V5 (many diffs) n/a
6 TRCN0000466664 ACTATCCCACAGTAGTGTCCTTCG pLX_317 21% 85.8% 82.2% V5 (many diffs) n/a
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