Construct: ORF TRCN0000466681
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014519.1_s317c1
- Derived from:
- ccsbBroadEn_01660
- DNA Barcode:
- CAAACCTTTCCCAAGCCTCCAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TFAP4 (7023)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466681
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7023 | TFAP4 | transcription factor AP-4 | NM_003223.3 | 100% | 100% | |
| 2 | human | 7023 | TFAP4 | transcription factor AP-4 | XM_011522633.3 | 94.4% | 90.4% | (many diffs) |
| 3 | human | 7023 | TFAP4 | transcription factor AP-4 | XM_011522635.3 | 82.2% | 82.2% | 0_1ins180 |
| 4 | mouse | 83383 | Tfap4 | transcription factor AP4 | NM_031182.2 | 91.2% | 97.6% | (many diffs) |
| 5 | mouse | 83383 | Tfap4 | transcription factor AP4 | XM_017317166.1 | 84.8% | 88% | (many diffs) |
| 6 | mouse | 83383 | Tfap4 | transcription factor AP4 | XM_006522762.3 | 74.4% | 79.8% | (many diffs) |
| 7 | mouse | 83383 | Tfap4 | transcription factor AP4 | XM_006522763.3 | 74.4% | 79.8% | (many diffs) |
| 8 | mouse | 83383 | Tfap4 | transcription factor AP4 | XM_006522764.3 | 74.4% | 79.8% | (many diffs) |
| 9 | mouse | 83383 | Tfap4 | transcription factor AP4 | XM_006522765.3 | 74.4% | 79.8% | (many diffs) |
| 10 | mouse | 83383 | Tfap4 | transcription factor AP4 | XM_006522766.3 | 74.4% | 79.8% | (many diffs) |
| 11 | mouse | 83383 | Tfap4 | transcription factor AP4 | XM_011246047.2 | 74.4% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1080
- ORF length:
- 1014
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtatttcatg gtgcccactc agaaggtgcc ctctttgcaa catttcagga 121 aaacagagaa agaagtgata ggagggctct gtagccttgc caacattcca ctaacccccg 181 agactcagcg ggaccaggag cggcggattc ggcgggagat cgccaacagc aacgagcgga 241 gacgcatgca gagcatcaac gcgggattcc agtccctcaa gaccctcatc ccccacacag 301 acggagagaa gctcagcaag gcagccattc tccagcagac agccgagtac atcttctccc 361 tggagcagga gaagaccagg ctcttgcagc agaacacaca gctcaagcgc ttcatccagg 421 agctgagcgg ctcgtccccc aagcgacggc gggcagagga caaggacgaa ggcataggct 481 ccccggacat ctgggaggac gagaaggcgg aggacctgcg gcgggagatg attgagctgc 541 ggcagcagct ggacaaggag cgctcggtgc gcatgatgct ggaggagcag gtgcgctcgc 601 tggaggccca catgtacccg gaaaagctca aggtgattgc gcagcaggtg cagctgcagc 661 agcagcagga acaggtgagg ctgctgcacc aggagaagct ggagcgggaa cagcagcagc 721 tgcggaccca gcttctgccc ccTCCGGCCC CCACCCACCA CCCCACGGTG ATCGTGCCAG 781 CACCGCCTCC TCCTCCCTCC CACCACATCA ATGTCGTCAC CATGGGCCCC TCCTCGGTCA 841 TCAACTCTGT TTCCACATCC CGGCAAAATC TGGACACCAT CGTGCAGGCA ATCCAGCACA 901 TCGAGGGCAC CCAGGAAAAG CAGGAGCTGG AGGAGGAGCA GCGGCGAGCT GTCATCGTGA 961 AGCCTGTCCG CAGCTGCCCG GAGGCCCCCA CCTCTGACAC CGCCTCCGAC TCCGAGGCCT 1021 CAGACAGTGA CGCCATGGAC CAGAGCCGGG AGGAGCCGTC GGGGGACGGG GAGCTTCCCT 1081 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1141 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1201 TTTATATATC TTGTGGAAAG GACGACAAAC CTTTCCCAAG CCTCCAATCA CGCGTTAAGT 1261 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt