Construct: ORF TRCN0000466698
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012645.1_s317c1
- Derived from:
- ccsbBroadEn_11842
- DNA Barcode:
- TCGAATGTTGACTGGTGAAGTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAU2 (27067)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466698
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164384.1 | 21.8% | 21.9% | 315_1437delinsA |
| 2 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_014393.2 | 21.8% | 21.9% | 315_1437delinsA |
| 3 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164385.1 | 20.5% | 20.6% | 0_1insATGCTTCAAATAAATCAG;297_1419delinsA |
| 4 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164381.1 | 19.4% | 19.5% | 315_1614delinsA |
| 5 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164380.2 | 17.8% | 17.5% | (many diffs) |
| 6 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001347045.1 | 20.5% | 21.9% | (many diffs) |
| 7 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_017321109.1 | 20.1% | 21.4% | (many diffs) |
| 8 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001347044.1 | 19% | 19.5% | (many diffs) |
| 9 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238390.2 | 18.6% | 19.1% | (many diffs) |
| 10 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001111272.1 | 18.2% | 19.5% | (many diffs) |
| 11 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_006495532.3 | 18.2% | 19.5% | (many diffs) |
| 12 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238389.1 | 18.2% | 19.5% | (many diffs) |
| 13 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238388.2 | 17.7% | 18.2% | (many diffs) |
| 14 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_025303.3 | 17% | 17.5% | (many diffs) |
| 15 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_006495528.3 | 17% | 17.5% | (many diffs) |
| 16 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238387.1 | 17% | 17.5% | (many diffs) |
| 17 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_017321102.1 | 17% | 17.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 91
- ORF end:
- 406
- ORF length:
- 315
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT 61 TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGCTTCAAA TAAATCAGAT GTTCTCAGTG 121 CAGCTGAGTC TTGGTGAGCA GACATGGGAA TCCGAAGGCA GCAGTATAAA GAAGGCTCAG 181 CAGGCTGTTG CCAATAAAGC TTTGACTGAA TCTACGCTTC CCAAACCAGT TCAGAAGCCA 241 CCCAAAAGTA ATGTTAACAA TAACCCAGGC AGTATAACTC CAACTGTGGA ACTGAATGGG 301 CTTGCTATGA AAAGGGGAGA GCCTGCCATC TACAGGCCAT TAGATCCAAA GCCATTCCCA 361 AATTATAGAG CTAATTACAA CTTTCGGGGC ATGTACAATC AGAGATGCCC AACTTTCTTG 421 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 481 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 541 GGAAAGGACG ATCGAATGTT GACTGGTGAA GTTATACGCG TTAAGTCgac aatcaacctc 601 tggattacaa aatttgtgaa agatt