Construct: ORF TRCN0000466716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016227.2_s317c1
- Derived from:
- ccsbBroadEn_15073
- DNA Barcode:
- CTTAACTCTCCCTAGTTGAACAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NLK (51701)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51701 | NLK | nemo like kinase | NM_016231.5 | 97.6% | 97.5% | 1_36del;73G>A |
| 2 | human | 51701 | NLK | nemo like kinase | XM_005257988.2 | 92.1% | 92% | 1_36del;73G>A;1149_1150ins87 |
| 3 | human | 51701 | NLK | nemo like kinase | XR_934482.1 | 72% | 1_263del;300G>A;1809_2144del | |
| 4 | human | 51701 | NLK | nemo like kinase | XR_001752526.2 | 62.6% | (many diffs) | |
| 5 | mouse | 18099 | Nlk | nemo like kinase | NM_008702.3 | 92.1% | 97.5% | (many diffs) |
| 6 | mouse | 18099 | Nlk | nemo like kinase | XR_001779914.1 | 31% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1611
- ORF length:
- 1545
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcttacaat ggcggtacat ctgcagcagc aacaggtcac caccaccacc 121 atcaccacca ccttccacac ctccctcctc ctcacctgca ccaccaccac caccctcaac 181 accatcttca tccggggtcg gctgccgctg tacaccctgt acagcagcac acctcttcgg 241 cagctgcggc agccgcagca gcggctgcag ctgcagccat gttaaaccct gggcaacaac 301 agccatattt cccatcaccg gcaccggggc aggctcctgg accagctgca gcagccccag 361 ctcaggtaca ggctgccgca gctgctacag ttaaggcgca ccatcatcag cactcgcatc 421 atccacagca gcagctggat attgagccgg atagacctat tggatatgga gcctttggtg 481 ttgtctggtc agtaacagat ccaagagatg gaaagagagt agcgctcaaa aagatgccca 541 acgtcttcca gaatctggtc tcttgcaaaa gggtcttccg ggaattgaag atgttgtgtt 601 tttttaagca tgataatgta ctctctgccc ttgacatact ccaacctcca cacattgact 661 attttgaaga aatatatgtt gtcacagaat tgatgcagag tgacctacat aaaattatcg 721 tctctcctca accactcagc tcagatcatg tcaaagtttt tctttatcag attttgcgag 781 gtttgaaata tctccattca gctggcattt tacatcgaga cattaagcca gggaatctcc 841 ttgtgaacag caactgtgtt ctaaagattt gtgattttgg attggccaga gtggaagaat 901 tagatgaatc ccgtcatatg actcaggaag ttgttactca gtattatcgg gctccagaaa 961 tcctgatggg cagccgtcat tacagcaatg ctattgacat ctggtctgtg ggatgtatct 1021 ttgcagaact actaggacga agaatattgt ttcaggcaca gagtcccatt cagcagttgg 1081 atttgatcac ggatctgttg ggcacaccat cactggaagc aatgaggaca gcttgtgaag 1141 gcgctaaggc acatatactc aggggtcctc ataaacagcc atctcttcct gtactctata 1201 ccctgtctag ccaggctaca catgaagctg ttcatctcct ttgcaggatg ttggtctttg 1261 atccatccaa aagaatatcc gctaaggatg ccttagccca cccctaccTA GATGAAGGGC 1321 GACTACGATA TCACACATGT ATGTGTAAAT GTTGCTTTTC CACCTCCACT GGAAGAGTTT 1381 ATACCAGTGA CTTTGAGCCT GTCACCAATC CCAAATTTGA TGACACTTTC GAGAAGAACC 1441 TCAGTTCTGT CCGACAGGTT AAAGAAATTA TTCATCAGTT CATTTTGGAA CAGCAGAAAG 1501 GAAACAGAGT GCCTCTCTGC ATCAACCCTC AGTCTGCTGC TTTTAAGAGC TTTATTAGTT 1561 CCACTGTTGC TCAGCCATCT GAGATGCCCC CATCTCCTCT GGTGTGGGAG TGCCCAACTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGACTTA ACTCTCCCTA GTTGAACAGG ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt