Construct: ORF TRCN0000466719
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018902.2_s317c1
- Derived from:
- ccsbBroadEn_14759
- DNA Barcode:
- TCTCCTTTCAGTAACGGCTAAGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PIK3CA (5290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466719
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | NM_006218.4 | 99.3% | 21.2% | (many diffs) |
| 2 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | XM_006713658.4 | 99.3% | 21.2% | (many diffs) |
| 3 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | XM_011512894.2 | 99.3% | 21.2% | (many diffs) |
| 4 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | NM_006218.2:c.3204_3205insA | 99.2% | 21.1% | (many diffs) |
| 5 | mouse | 18706 | Pik3ca | phosphatidylinositol-4,5-bi... | NM_008839.2 | 89.1% | 20.8% | (many diffs) |
| 6 | mouse | 18706 | Pik3ca | phosphatidylinositol-4,5-bi... | XM_006535409.3 | 89.1% | 20.8% | (many diffs) |
| 7 | mouse | 18706 | Pik3ca | phosphatidylinositol-4,5-bi... | XM_006535410.3 | 89.1% | 20.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 774
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctccacga ccatcatcag gtgaactgtg gggcatccac ttgatgcccc 121 caagaatcct agtagaatgt ttactaccaa atggaatgat agtgacttta gaatgcctcc 181 gtgaggctac attaataacc ataaagcatg aactatttaa agaagcaaga aaataccccc 241 tccatcaact tcttcaagat gaatcttctt acattttcgt aagtgttact caagaagcag 301 aaagggaaga attttttgat gaaacaagac gactttgtga ccttcggctt tttcaaccct 361 ttttaaaagt aattgaacca gtaggcaacc gtgaagaaaa gatcctcaat cgagaaattg 421 gttttgctat cggcatgcca gtgtgtgaat ttgatatggt taaagatcca gaagtacagg 481 acttccgaag aaatattctg aacgtttgta aagaagctgt ggatcttagg gacctcaatt 541 cacctcatag tagagcaatg tatgtctatc ctccaaatgt agaatcttca ccagaattgc 601 caaagcacat atataataaa ttagataaag ggcaaataat agtggtgatc tgggtaatag 661 tttctccaaa taatgacaag cagaagtata ctctgaaaat caaccatgac tgtgtaccag 721 aacaagtaat tgctgaagca atcagaaaaa actcgaagta tgttgctatc ctctgacact 781 aaactctgtg ttttagatat cagcaagtat attttaaagt gtgtggatgt gatgaatact 841 tcctagaaaa atatcctctg agtcagtata agtatataag aagctgtata atgcttggga 901 ggatgcccaa tttgatgttg atggctaaag aaagccttta ttctcaactg ccaatggact 961 gttttacaat gccatcttat tccagacgca tttccacagc tacaccatat atgaatggag 1021 aaacatctac aaaatccctt tgggttataa atagtgcact cagaataaaa attctttgtg 1081 caacctacgt gaatgtaaat attcgagaca ttgataagat ctatgttcga acaggtatct 1141 accatggagg agaaccctta tgtgacaatg tgaacactca aagagtacct tgttccaatc 1201 ccaggtggaa tgaatggctg aattatgata tatacattcc tgatcttcct cgtgctgctc 1261 gactttgcct ttccatttgc tctgttaaag gccgaaaggg tgctaaagag gaacactgtc 1321 cattggcatg gggaaatata aacttgtttg attacacaga cactctagta tctggaaaaa 1381 tggctttgaa tctttggcca gtacctcatg gattagaaga tttgctgaac cctattggtg 1441 ttactggatc aaatccaaat aaagaaactc catgcttaga gttggagttt gactggttca 1501 gcagtgtggt aaagttccca gatatgtcag tgattgaaga gcatgccaat tggtctgtat 1561 cccgagaagc aggatttagc tattcccacg caggactgag taacagacta gctagagaca 1621 atgaattaag ggaaaatgac aaagaacagc tcaaagcaat ttctacacga gatcctctct 1681 ctgaaatcac tgagcaggag aaagattttc tatggagtca cagacactat tgtgtaacta 1741 tccccgaaat tctacccaaa ttgcttctgt ctgttaaatg gaattctaga gatgaagtag 1801 cccagatgta ttgcttggta aaagattggc ctccaatcaa acctgaacag gctatggaac 1861 ttctggactg taattaccca gatcctatgg ttcgaggttt tgctgttcgg tgcttggaaa 1921 aatatttaac agatgacaaa ctttctcagt atttaattca gctagtacag gtcctaaaat 1981 atgaacaata tttggataac ttgcttgtga gatttttact gaagaaagca ttgactaatc 2041 aaaggattgg gcactttttc ttttggcatt taaaatctga gatgcacaat aaaacagtta 2101 gccagaggtt tggcctgctt ttggagtcct attgtcgtgc atgtgggatg tatttgaagc 2161 acctgaatag gcaagtcgag gcaatggaaa agctcattaa cttaactgac attctcaaac 2221 aggagaagaa ggatgaaaca caaaaggtac agatgaagtt tttagttgag caaatgaggc 2281 gaccagattt catggatgct ctacagggct ttctgtctcc tctaaaccct gctcatcaac 2341 taggaaacct caggcttgaa gagtgtcgaa ttatgtcctc tgcaaaaagg ccactgtggt 2401 tgaattggga gaacccagac atcatgtcag agttactgtt tcagaacaat gagatcctct 2461 ttaaaaatgg ggatgattta cgccaagata tgctaacact tccaanttat tcgtatatgg 2521 gaaaatatct gcaaaaatca aggtcntgat cttcgaatgt taccttatgg ttgtctgtca 2581 atcggtgact gtgtgggact tatgaggtgg gtgcgaaatt ctcacactat tatgcaaatt 2641 cagtgcaaag gcggcttgaa aggtgcactg cagttcaaca gccacacact acatcagtgg 2701 ctcaaagaca agaacaaagg agaaatatat gatgcagcca ttgacctgtt tacacgttca 2761 tgtgctggat actgtgtagc taccttcatt ttgggaattg gagatcgtca caatagtaac 2821 atcatggtga aagacgatgg acaactgttt catatagatt ttggacactt tttggatcac 2881 aagaagaaaa aatttggtta taaacgagaa cgtgtgccat ttgttttgac acaggattTC 2941 TTAATAGTGA TTAGTAAAGG AGCCCAAGAA TGCACAAAGA CAAGAGAATT TGAGAGGTTT 3001 CAGGAGATGT GTTACAAGGC TTATCTAGCT ATTCGACAGC ATGCCAATCT CTTCATAAAT 3061 CTTTTCTCAA TGATGCTTGG CTCTGGAATG CCAGAACTAC AATCTTTTGA TGACATTGCA 3121 TACATTCGAA AGACCCTAGC CTTAGATAAA ACTGAGCAAG AGGTTTTGGA GTATTTCATG 3181 AAACAAATGA ATGATGCACA TCATGGTGGC TGGACAACAA AAATGGATTG GATCTTCCAC 3241 ACAATTAAAC AGCATGCATT GAACTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT 3301 AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA 3361 CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCTCCTTTCA 3421 GTAACGGCTA AGACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 3481 gatt