Construct: ORF TRCN0000466755
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003655.1_s317c1
- Derived from:
- ccsbBroadEn_11651
- DNA Barcode:
- TGAGAGTTTTCAACTTTGCGAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF6 (22926)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466755
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22926 | ATF6 | activating transcription fa... | XM_011509310.2 | 33.5% | 33.1% | (many diffs) |
| 2 | human | 22926 | ATF6 | activating transcription fa... | XM_006711224.1 | 29.7% | 29.4% | (many diffs) |
| 3 | human | 22926 | ATF6 | activating transcription fa... | NM_007348.4 | 29.7% | 29.4% | (many diffs) |
| 4 | human | 22926 | ATF6 | activating transcription fa... | XM_011509309.1 | 28.9% | 28.6% | (many diffs) |
| 5 | human | 22926 | ATF6 | activating transcription fa... | XM_011509308.1 | 28.9% | 28.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 672
- ORF length:
- 606
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggagccggct ggggttgccg gcaccatgga gtcacctttt agcccgggac 121 tctttcacag gctggatgaa gattgggatt ctgctctctt tgctgaactc ggttatttca 181 cagacactga tgagctgcaa ttggaagcag caaatgagac gtatgaaaac aattttgata 241 atcttgattt tgatttggat ttggtgcctt gggagtcaga cattTGGGAC ATCAACAACC 301 AAATCTGTAC AGTTAAAGAT ATTAAGGCAG AACCTCAGCC ACTTTCTCCA GCCTCCTCAA 361 GTTATTCAGT CTCGTCTCCT CGGTCAGTGG ACTCTTATTC TTCAACTCAG CATGTTCCTG 421 AGGAGTTGGA TTTGTCTTCT AGTTCTCAGA TGTCTCCCCT TTCCTTATAT GGTGAAAACT 481 CTAATAGTCT CTCTTCAGCG GAGCCACTGA AGGAAGATAA GCCTGTCACT GGTCCTAGGA 541 ACAAGACTGA AAATGGACTG ACTCCAAAGA AAAAAATTCA GGTGAATTCA AAACCTTCAA 601 TTCAGCCCAA GCCTTTATTG CTTCCAGCAG CACCCAAGAC TCAAACAATC TCCAGCATTC 661 CCCCTCAAAC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 721 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 781 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGA GAGTTTTCAA CTTTGCGAAC 841 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t