Construct: ORF TRCN0000466760
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002459.1_s317c1
- Derived from:
- ccsbBroadEn_07504
- DNA Barcode:
- GAGACAGTCGAGCAACCATATACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZBED4 (9889)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466760
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9889 | ZBED4 | zinc finger BED-type contai... | NM_014838.3 | 99.7% | 99.8% | (many diffs) |
| 2 | human | 9889 | ZBED4 | zinc finger BED-type contai... | XM_024452312.1 | 99.7% | 99.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3582
- ORF length:
- 3513
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaataac ttgaaaactt gtcccaaaga ggacggtgat ttcgtttctg 121 ataaaataaa gtttaaaata gaagaggaag atgatgatgg aattcctcct gatagtttgg 181 aaagaatgga ctttaaaagc gagcaggagg acatgaagca gacagacagc ggtggggagc 241 gagcgggcct cggtgggacg ggttgcagct gcaagccccc tgggaagtac ttgtctgcag 301 agagtgagga tgactatggc gcgctgttct cccagtacag cagcacccta tacgacgtgg 361 ccatggaggc cgtgacccag agcctccttt ccagccggaa catgagctcc aggaagaagt 421 ctccagcctg gaagcatttt tttatctctc cccgagacag cactaaagca atatgcatgt 481 actgtgtgaa ggagttcagc agaggcaaaa acgagaaaga cttgagtacc agttgtctca 541 tgaggcacgt gaggcgcgca cacccgaccg tgctcattca ggaaaatggc agcgtgtctg 601 ccgtgtcctc gttcccctct ccctcactcc tgcttccacc gcagcctgcg gacgcgggtg 661 acctcagcac catcctctca cccatcaaac ttgtccagaa agtggcgtct aagatcccgt 721 cccccgatcg aataacagag gagtctgtgt ctgtagtttc ttctgaagaa atctcctctg 781 acatgtccgt ttcggagaag tgcggcagag aagaagccct ggtggggtcg tctccccacc 841 tccctgctct ccattacgat gaacctgcag agaacttagc ggagaagagc cttccacttc 901 caaagagcac ctctgggtcc aggagaaggt ccgctgtctg gaagcacttc tacctgtcgc 961 cactggacaa ctccaaagct gtctgcattc actgcatgaa cgagttcagc cgggggaaga 1021 atgggaagga cctgggcacg agctgcctca tcaggcacat gtggagggca caccgcgcca 1081 tcgtgttgca ggagaacggg ggcacgggca tcccgccact gtactccacc cctcccactc 1141 tgctgccttc cttgctgccg ccggaggggg agctcagctc tgtgtcctcg tctccggtaa 1201 agccggtcag agagtcccct tcggcctcct cctcccctga caggctgact gaggacttgc 1261 agtctcactt gaaccctgga gatgggctga tggaagacgt ggcggccttc tcatcttccg 1321 atgacgtagg ggaggcctcg gcgtcctctc ctgagaagca gcaggctgat gggctgagtc 1381 ctagattgtt tgaatctggt gccatcttcc agcagaataa aaaggtcatg aaaagactga 1441 agtcggaggt ctggcatcac ttctccctgg cccccatgga cagcctcaag gccgagtgtc 1501 ggtactgcgg ctgtgccatc agccggggga agaagggtga tgtgggcacc agctgcctga 1561 tgagacatct ctaccggcgc catccagaag ttgtcgggag ccagaagggc ttcctgggtg 1621 caagtctggc aaactctccg tatgccactt tggcctctgc agaaagttcc tcttccaaat 1681 tgactgactt gccaacagtg gtcacaaaaa acaatcaagt tatgtttcct gttaatagca 1741 aaaagacctc gaagctgtgg aatcattttt ctatttgctc cgcagactcc acaaaagtcg 1801 tgtgcttgca ctgtggccag accatcagcc gggggaagaa gccgactaac ttgggtacca 1861 gctgtcttct gagacattta cagcggttcc atagcaacgt gctgaaaact gaggtctcgg 1921 agacggctcg gccctcctct ccggacaccc gggtgccgcg gggcacagaa ttatcaggcg 1981 cttcctcttt tgatgacacc aatgagaagt tttacgattc tcacccagtt gccaaaaaaa 2041 tcacaagtct catagctgaa atgattgcac ttgacctcca gccatattct tttgtagaca 2101 acgttggctt taacaggctg cttgaatact tgaaacctca gtactccctc cccgcccctt 2161 cctacttctc caggacagct atcccaggta tgtatgataa tgtgaagcag ataattatgt 2221 cccaccttaa ggaagctgag agtggtgtga tccacttcac gtctggaata tggatgagta 2281 accagacccg tgagtacctg accctcacgg cccactgggt ttccttcgag tcgccagccc 2341 ggccgcgctg tgacgaccac cactgctcgg cgctgttgga cgtgtcgcag gtggactgcg 2401 actacagtgg caacagcatt cagaagcagc tggagtgctg gtgggaagcg tgggtgacct 2461 ccaccggcct tcaggtgggc atcaccgtca ccgacaacgc cagcatcggg aagacgctga 2521 acgaggggga gcactcgagc gtgcagtgct tcagccatac ggtgaacctg atcgtcagcg 2581 aggccattaa gagccagcgg atggtgcaga acctgctgag cctcgcccgg aagatctgcg 2641 agcgggtgca ccggtcgccc aaggcgaagg agaaactggc cgagctgcag agggagtacg 2701 cgctgcctca gcatcacctc atccaggacg tcccgtccaa gtggagcact tccttccaca 2761 tgctcgagcg gctcattgag cagaaaaggg ccattaacga gatgtccgtc gagtgtaact 2821 tccgagagct gatcagctgc gaccagtggg aggtcatgca gtccgtgtgc cgtgcgctaa 2881 agcccttcga ggccgcgagc cgggagatga gcacgcagat gtccaccctc agccaggtca 2941 tccccatggt acacatcctc aacaggaagg tggagatgct cttcgaggag acgatgggca 3001 tcgacaccat gctgcgctct ctgaaggagg ccatggtgag ccgcctgtct gccaccctcc 3061 acgacccgcg gtacgtcttc gccacgctgc tggatcctcg ctacaaggcc tccctgttta 3121 cggaggagga ggcggagcag tacaaacagg atttaatcag ggaactcgaa ctcatgaatt 3181 ctacctcaga ggacgtggct gccTCCCACA GGTGTGATGC TGGCTCCCCG TCGAAAGACT 3241 CTGCCGCAGA GGAGAACCTG TGGTCACTTG TGGCCAAGGT GAAGAAGAAA GACCCAAGAG 3301 AAAAGCTGCC TGAAGCCATG GTGCTTGCGT ATCTGGAGGA GGAGGTGCTT GAACACAGCT 3361 GTGACCCGCT CACCTACTGG AACCTGAAGA AGGCGTCCTG GCCGGGGCTG TCCGCGCTGG 3421 CCGTCAGATT TTTGGGCTGC CCCCCAAGCA TCGTCCCTTC AGAAAAGCTG TTCAACACAC 3481 CCACTGAGAA CGGTAGCCTT GGCCAATCCA GGCTCATGAT GGAACATTTT GAAAAACTTA 3541 TCTTTTTGAA AGTGAATCTT CCCTTAATAT ACTTTCAGTA TTTGCCAACT TTCTTGTACA 3601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3721 AGGACGAGAG ACAGTCGAGC AACCATATAC AACGCGTTAA GTCgacaatc aacctctgga 3781 ttacaaaatt tgtgaaagat t