Construct: ORF TRCN0000466764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014494.1_s317c1
- Derived from:
- ccsbBroadEn_00034
- DNA Barcode:
- TGTCTCCGGCCTGGGCGCCTTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TLE5 (166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466764
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 166 | TLE5 | TLE family member 5, transc... | NM_198969.1 | 100% | 100% | |
| 2 | human | 166 | TLE5 | TLE family member 5, transc... | XM_006722664.1 | 99.6% | 99.6% | 573_574insCAG |
| 3 | human | 166 | TLE5 | TLE family member 5, transc... | NM_001130.6 | 72.3% | 71.5% | (many diffs) |
| 4 | human | 166 | TLE5 | TLE family member 5, transc... | NM_198970.2 | 72% | 71.2% | (many diffs) |
| 5 | mouse | 14797 | Aes | amino-terminal enhancer of ... | NM_010347.4 | 63.2% | 71.2% | (many diffs) |
| 6 | mouse | 14797 | Aes | amino-terminal enhancer of ... | NM_001276288.1 | 62.8% | 70.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 861
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgtcacaag aatggcttcc ctcaggaagg cggcattacc gctgcctttc 121 tgcagaaaag gaaactaagg ctcagcaaga accaccgccc agccagagcc aaggtcacag 181 agcacgtccg tggcacgcgt ccaggtcgtg ccacagcagg gccggcggct tcgacgcggg 241 cagccgggtc ccttttcttt gacagatggg gaaaccgagg cccggccggc tgccggggct 301 cctcgcacct accccagcaa ctcaaattca ccacctcgga ctcctgcgac cgcatcaaag 361 acgaatttca gctactgcaa gctcagtacc acagcctcaa gctcgaatgt gacaagttgg 421 ccagtgagaa gtcagagatg cagcgtcact atgtgatgta ctacgagatg tccTACGGCT 481 TGAACATCGA GATGCACAAA CAGGCTGAGA TCGTCAAAAG GCTGAACGGG ATTTGTGCCC 541 AGGTCCTGCC CTACCTCTCC CAAGAGCACC AGCAGCAGGT CTTGGGAGCC ATTGAGAGGG 601 CCAAGCAGGT CACCGCTCCC GAGCTGAACT CTATCATCCG ACAGCAGCTC CAAGCCCACC 661 AGCTGTCCCA GCTGCAGGCC CTGGCCCTGC CCTTGACCCC ACTACCCGTG GGGCTGCAGC 721 CGCCTTCGCT GCCGGCGGTC AGCGCAGGCA CCGGCCTCCT CTCGCTGTCC GCGCTGGGTT 781 CCCAGGCCCA CCTCTCCAAG GAAGACAAGA ACGGGCACGA TGGTGACACC CACCAGGAGG 841 ATGATGGCGA GAAGTCGGAT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 901 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 961 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGTC TCCGGCCTGG 1021 GCGCCTTTCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt