Construct: ORF TRCN0000466769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016259.1_s317c1
- Derived from:
- ccsbBroadEn_08998
- DNA Barcode:
- GCAAAGACAAACACCGCACCCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGLUCY (80017)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466769
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001102366.2 | 99.9% | 100% | 306C>T |
| 2 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001102367.2 | 99.9% | 100% | 306C>T |
| 3 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001286471.1 | 99.9% | 100% | 306C>T |
| 4 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001358311.1 | 99.9% | 100% | 306C>T |
| 5 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_024952.7 | 99.9% | 100% | 306C>T |
| 6 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001102368.2 | 99.1% | 99.1% | 306C>T;440_454del |
| 7 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001286470.1 | 99.1% | 99.1% | 306C>T;440_454del |
| 8 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001358310.1 | 99.1% | 99.1% | 306C>T;440_454del |
| 9 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001358312.1 | 99.1% | 99.1% | 306C>T;440_454del |
| 10 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001286472.1 | 92.6% | 92.7% | 306C>T;440_454del;1441_1442ins120 |
| 11 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001102369.2 | 91.5% | 91.5% | 306C>T;554_555ins36;1390_1391ins120 |
| 12 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001286473.2 | 22.8% | 15.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1914
- ORF length:
- 1848
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cttcacactc cacctgaggt cccgccttcc ctctgccata aggagtttga 121 ttctacaaaa gaaaccaaac atcagaaata catccagcat ggctggagag ctccgaccag 181 ccagcctggt ggtcctgccc aggtcccttg ctccagcttt tgaaagattc tgccaggtca 241 acactggtcc tctacccctg ctgggccaga gtgagccaga aaagtggatg ctgccccctc 301 aaggtgctat ctcagagacc aggatgggcc atccccagtt ctggaaatac gagttcggtg 361 cctgcaccgg tagcctggct tcgctggagc agtactcgga gcagctgaag gacatggtgg 421 ccttcttcct gggctgcagc ttctccctgg aggaggcctt ggagaaagcg gggctcccca 481 gaagagaccc agcaggtcac agccagacaa cagtgccttg tgttacccat gctggcttct 541 gctgccctct ggtggtcacg atgaggccca ttcccaagga caagctggaa gggctggtgc 601 gggcctgctg ctccctcgga ggtgagcagg ggcaacctgt tcacatgggc gacccagaac 661 tgttgggaat caaagagctt tccaaacctg cctacgggga tgccatggtg tgtcccccag 721 gggaggttcc agtgttctgg ccttctccgc tgaccagtct cggagctgtc agcagctgtg 781 agaccccact ggcttttgcc agcatcccag gctgcacagt tatgactgac ctgaaggatg 841 caaaggctcc acctggttgt ctcaccccag agagaattcc agaggtccat cacatttccc 901 aagatcctct gcactacagc atcgcgtcag tctctgcttc tcagaagatc agagaactag 961 agtctatgat cggcatagac ccagggaacc gggggattgg gcacctgctc tgtaaagatg 1021 agctgctgaa ggcctctctc tcgctgtccc atgcccgctc agtgctcatc accactgggt 1081 tccccacaca tttcaatcat gagcctccag aagagacaga tggcccacca ggagctgttg 1141 ctctggttgc cttcctgcag gccttggaga aggaggtcgc cataatcgtt gaccagagag 1201 cctggaactt gcaccagaag attgttgaag atgctgttga gcaaggtgtt ctgaagacgc 1261 agatcccgat attaacttac caaggtggat cagtggaagc tgctcaggca ttcctgtgca 1321 aaaatgggga cccgcagaca cctagatttg accacctggt ggccatagag cgtgccggaa 1381 gagctgctga tggcaattac tacaatgcaa ggaagatgaa catcaagcac ttggttgacc 1441 ccattgacga tctttttctt gctgcgaaga agattcctgg aatctcatca actggagtcg 1501 gtgatGGAGG CAACGAGCTT GGGATGGGTA AAGTCAAGGA GGCTGTGAGG AGGCACATAC 1561 GGCACGGGGA TGTCATCGCC TGCGACGTGG AGGCTGACTT TGCCGTCATT GCTGGTGTTT 1621 CTAACTGGGG AGGCTATGCC CTGGCCTGCG CACTCTACAT CCTGTACTCA TGTGCTGTCC 1681 ACAGTCAGTA CCTGAGGAAA GCAGTCGGAC CCTCCAGGGC ACCTGGAGAT CAGGCCTGGA 1741 CTCAGGCCCT CCCGTCGGTC ATTAAGGAAG AAAAAATGCT GGGCATCTTG GTGCAGCACA 1801 AAGTCCGGAG TGGCGTCTCG GGCATCGTGG GCATGGAGGT GGATGGGCTG CCCTTCCACA 1861 ACACCCACGC CGAGATGATC CAGAAGCTGG TGGACGTCAC CACGGCACAG GTGTACCCAA 1921 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1981 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2041 TATCTTGTGG AAAGGACGAG CAAAGACAAA CACCGCACCC CCCACGCGTT AAGTCgacaa 2101 tcaacctctg gattacaaaa tttgtgaaag att