Construct: ORF TRCN0000466783
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000053.1_s317c1
- Derived from:
- ccsbBroadEn_11258
- DNA Barcode:
- AGCCCGAATAGGCTCTTACCAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELL (8178)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466783
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8178 | ELL | elongation factor for RNA p... | XR_001753769.2 | 6.3% | 1_3311del;3576_4165del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 330
- ORF length:
- 264
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ttGGAAAGGT GGTGGGGGGT GGATGGCGGC TGTGACTCAG GGCCCAGGGA 121 TCACATGGGG GTCACTGCTG CCATCAGCAG TCAGGCAGGG AGAACAGAAA TGCAAAAATA 181 CAGATGTCTA TTTTTCTAAA CTGGGGGGTG GAGGGGTGCC TCCTGACAGC TTCCAAAGAG 241 CTGACTGTGG AGGGCCCACA GTGTATCCCA CCCCTGCCCG AGAGTCTCTC TCCTTTGGGA 301 CCCCCGGGTT CCCAGAGCTC CGAGGAAACT GCCCAACTTT CTTGTACAAA GTGGTTGATA 361 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 421 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGCCC 481 GAATAGGCTC TTACCAAGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 541 tgaaagatt