Construct: ORF TRCN0000466793
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007434.1_s317c1
- Derived from:
- ccsbBroadEn_06280
- DNA Barcode:
- ATTACATATTATCTTGTGATCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPC3 (2719)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466793
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2719 | GPC3 | glypican 3 | NM_004484.4 | 99.9% | 99.8% | 1076C>T |
| 2 | human | 2719 | GPC3 | glypican 3 | NM_001164618.2 | 97.1% | 96.8% | 289_290ins48;1028C>T |
| 3 | human | 2719 | GPC3 | glypican 3 | NM_001164617.2 | 96.1% | 96% | 1033_1101del;1145C>T |
| 4 | human | 2719 | GPC3 | glypican 3 | NM_001164619.2 | 90.6% | 90.5% | 173_174ins162;914C>T |
| 5 | human | 2719 | GPC3 | glypican 3 | XM_017029413.2 | 78.5% | 76.5% | (many diffs) |
| 6 | mouse | 14734 | Gpc3 | glypican 3 | NM_016697.3 | 90.5% | 93.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1806
- ORF length:
- 1740
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgggaccgtg cgcaccgcgt gcttggtggt ggcgatgctg ctcagcttgg 121 acttcccggg acaggcgcag cccccgccgc cgccgccgga cgccacctgt caccaagtcc 181 gctccttctt ccagagactg cagcccggac tcaagtgggt gccagaaact cccgtgccag 241 gatcagattt gcaagtatgt ctccctaagg gcccaacatg ctgctcaaga aagatggaag 301 aaaaatacca actaacagca cgattgaaca tggaacagct gcttcagtct gcaagtatgg 361 agctcaagtt cttaattatt cagaatgctg cggttttcca agaggccttt gaaattgttg 421 ttcgccatgc caagaactac accaatgcca tgttcaagaa caactaccca agcctgactc 481 cacaagcttt tgagtttgtg ggtgaatttt tcacagatgt gtctctctac atcttgggtt 541 ctgacatcaa tgtagatgac atggtcaatg aattgtttga cagcctgttt ccagtcatct 601 atacccagct aatgaaccca ggcctgcctg attcagcctt ggacatcaat gagtgcctcc 661 gaggagcaag acgtgacctg aaagtatttg ggaatttccc caagcttatt atgacccagg 721 tttccaagtc actgcaagtc actaggatct tccttcaggc tctgaatctt ggaattgaag 781 tgatcaacac aactgatcac ctgaagttca gtaaggactg tggccgaatg ctcaccagaa 841 tgtggtactg ctcttactgc cagggactga tgatggttaa accctgtggc ggttactgca 901 atgtggtcat gcaaggctgt atggcaggtg tggtggagat tgacaagtac tggagagaat 961 acattctgtc ccttgaagaa cttgtgaatg gcatgtacag aatctatgac atggagaacg 1021 tactgcttgg tctcttttca acaatccatg attctatcca gtatgtccag aagaatgcag 1081 gaaagctgac caccactatt ggcaagttat gtgcccattc tcaacaacgc caatatagat 1141 ttgcttatta tcctgaagat ctctttattg acaagaaagt attaaaagtt gctcatgtag 1201 aacatgaaga aaccttatcc agccgaagaa gggaactaat tcagaagttg aagtctttca 1261 tcagcttcta tagtgctttg cctggctaca tctgcagcca tagccctgtg gcggaaaacg 1321 acaccctttg ctggaatgga caagaactcg tggagagata cagccaaaag gcagcaagga 1381 atggaatgaa aaaccagttc aatctccatg agctgaaaat gaagggcccT GAGCCAGTGG 1441 TCAGTCAAAT TATTGACAAA CTGAAGCACA TTAACCAGCT CCTGAGAACC ATGTCTATGC 1501 CCAAAGGTAG AGTTCTGGAT AAAAACCTGG ATGAGGAAGG GTTTGAAAGT GGAGACTGCG 1561 GTGATGATGA AGATGAGTGC ATTGGAGGCT CTGGTGATGG AATGATAAAA GTGAAGAATC 1621 AGCTCCGCTT CCTTGCAGAA CTGGCCTATG ATCTGGATGT GGATGATGCG CCTGGAAACA 1681 GTCAGCAGGC AACTCCGAAG GACAACGAGA TAAGCACCTT TCACAACCTC GGGAACGTTC 1741 ATTCCCCGCT GAAGCTTCTC ACCAGCATGG CCATCTCGGT GGTGTGCTTC TTCTTCCTGG 1801 TGCACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1861 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1921 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATTACATAT TATCTTGTGA TCTCAACGCG 1981 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt