Construct: ORF TRCN0000466850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000758.1_s317c1
- Derived from:
- ccsbBroadEn_10223
- DNA Barcode:
- AGGCGCTCCGGATATCTATCGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2B7P (1556)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466850
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1555 | CYP2B6 | cytochrome P450 family 2 su... | NM_000767.5 | 73.3% | 70% | (many diffs) |
| 2 | human | 1556 | CYP2B7P | cytochrome P450 family 2 su... | NR_001278.1 | 37.7% | 1_8delCTGGAACC;1140_3000del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctcagcgtc ctcctcttcc ttgcactcct cacaggcctc ttgctactcc 121 tggttcagcg tcaccctaac tcccatggca ccctcccacc agggccccgc cctctgcccc 181 ttttggggaa ccttctgcag atggacagaa gaggcctact caaatccttt ctgaggttcc 241 gagagaaata tggggacgtc ttcacggtac acctgggacc gaggcccgtg gtcatgctgt 301 gtggagtaga ggccatacgg gaggccctgg tggacaacgc tgaggccttc tctggccggg 361 gaaaaatcgt catcatggac ccagtctacc agggatatgg catgctcttt gccaatggaa 421 accgctggaa ggtgcttcgg cgattctctg tgaccaccat gagggacttc gggatgggaa 481 agcggagtgt ggaggagcgg attcaggacg aggctcagtg tctgatagag gaacttcgga 541 aatccaaggg agccctcgtg gaccccacct tcctcttcca ttccattacc gccaacatca 601 tctgctccat catctttgga aaacgcttcc actaccaaga tcaagagttc ctgaagacgc 661 tgaacttgtt ctgccagagt ttcttactca tcagctctat atccagccag ctgtttgagc 721 tcttctctgg cttcttgaaa tactttcctg gggcacacag gcaagtttac aaaaaccTAC 781 AGGAAATCAA TGCTTACATT GGCCACAGTG TGGAGAAGCA CCGTGAAACC CTGGACCCCA 841 GCGCCCCCAG GGACCTCATC GACACCTACC TGCTCCACAT GGAAAAAGAG AAATCCAACC 901 CACACAGTGA ATTCAGCCAC CAGAACCTCA TCATCAACAC GCTCTCGCTC TTCTTTGCTG 961 GCACTGAGAC CACCAGCACC ACTCTCCGCT ACGGCTTCCT GCTCATGCTC AAATACCCTC 1021 ATGTCGCAGA GAGAGTCTAC AAGGAGATTG AACAGGTGGT TGGCCCACAT CGCCCTCCAG 1081 CGCTTGATGA CCGAGCCAAA ATGCCATACA CAGAGGCAGT CATCCGTGAG ATTCAGAGAT 1141 TTGCTGACCT TCTCCCCATG GGTGTGCCCC ACATTGTCAC CCAACACACC AGCTTCTGCC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GAAGGCGCTC CGGATATCTA TCGGCCACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt