Construct: ORF TRCN0000466868
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007244.1_s317c1
- Derived from:
- ccsbBroadEn_03784
- DNA Barcode:
- TCACATTCTACTCATCCACCATGC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB25 (57111)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466868
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57111 | RAB25 | RAB25, member RAS oncogene ... | NM_020387.4 | 100% | 100% | |
| 2 | human | 57111 | RAB25 | RAB25, member RAS oncogene ... | NR_133653.2 | 55.7% | (many diffs) | |
| 3 | mouse | 53868 | Rab25 | RAB25, member RAS oncogene ... | NM_016899.4 | 90.6% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaatggaact gaggaagatt ataactttgt cttcaaggtg gtgctgatcg 121 gcgaatcagg tgtggggaag accaatctac tctcccgatt cacgcgcaat gagttcagcc 181 acgacagccg caccaccatc ggggttgagt tctccacccg cactgtgatg ttgggcaccg 241 ctgctgtcaa ggctcagatc tgggacacag ctggcctgga gcggtaccga gccatcacct 301 cggcgtacta tcgtggtgca gtgggggccc tcctggtgtt TGACCTAACC AAGCACCAGA 361 CCTATGCTGT GGTGGAGCGA TGGCTGAAGG AGCTCTATGA CCATGCTGAA GCCACGATCG 421 TCGTCATGCT CGTGGGTAAC AAAAGTGACC TCAGCCAGGC CCGGGAAGTG CCCACTGAGG 481 AGGCCCGAAT GTTCGCTGAA AACAATGGAC TGCTCTTCCT GGAGACCTCA GCCCTGGACT 541 CTACCAATGT TGAGCTAGCC TTTGAGACTG TCCTGAAAGA AATCTTTGCG AAGGTGTCCA 601 AGCAGAGACA GAACAGCATC CGGACCAATG CCATCACTCT GGGCAGTGCC CAGGCTGGAC 661 AGGAGCCTGG CCCTGGGGAG AAGAGGGCCT GTTGCATCAG CCTCGCCCAA CTTTCTTGTA 721 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 781 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 841 AAAGGACGAT CACATTCTAC TCATCCACCA TGCACGCGTT AAGTCgacaa tcaacctctg 901 gattacaaaa tttgtgaaag att