Construct: ORF TRCN0000466871
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012679.1_s317c1
- Derived from:
- ccsbBroadEn_10364
- DNA Barcode:
- ACAGCGAGTAGACGACGTGCACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGF7P6 (387628)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466871
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 403323 | LERFS | lncRNA negative regulator o... | NR_122077.1 | 44% | (many diffs) | |
| 2 | human | 403323 | LERFS | lncRNA negative regulator o... | NR_122076.1 | 28.9% | (many diffs) | |
| 3 | human | 387628 | FGF7P6 | fibroblast growth factor 7 ... | NR_003674.2 | 21.7% | 1_76del;536_2115del | |
| 4 | human | 387628 | FGF7P6 | fibroblast growth factor 7 ... | NR_047527.1 | 20.6% | 1_76del;536_2225del | |
| 5 | human | 654466 | FGF7P3 | fibroblast growth factor 7 ... | NR_003670.1 | 9.7% | 1_175del;635_4723del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 528
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtggattc gggtccagat tctggcagga gggagtttgg gatcgagatc 121 tggaaaaaag cactagactg gaagaggacg cgatGGAGTC GGAGCCGCTG GCGGGTACAA 181 AAACCAGAGG CCGGGGAAGG CGCCGGTGGG AGGCAAGGCA CGGATGGACT TTACCTGCGC 241 ACGCGTCGCA GCCATCTCCG CGCACAGTGG TGGCCACCGC GACTGGTGCT GAAGTGTCGG 301 CGTGTGCCGG GCGCTCCGCT GGGACCCGGG TTGCTCGCCC TGAGTCTCAG CTTTCTCATC 361 TGTACGGTTG GGACAAGTAC AGTAACCCTC GCCCGTCAAG ACGGGCCAGG GCTGTGGCGA 421 GGGTCCACGC CTTAGAGCAG GCACCTATCT TGTGCAGGGC CCTGAGATGG GGTCTGACTC 481 AGTTCCTGCG GGGAACTTCA CCAGTGACCC AGTCAGTGCC CTTCAGTTTG CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGAACAGCGA GTAGACGACG TGCACTTACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt