Construct: ORF TRCN0000466886
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008745.2_s317c1
- Derived from:
- ccsbBroadEn_15292
- DNA Barcode:
- AGCTTAAAAACTGCCTTGGATCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK15 (225689)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466886
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_011516928.2 | 71.3% | 68.7% | (many diffs) |
| 2 | human | 225689 | MAPK15 | mitogen-activated protein k... | NM_139021.3 | 46.2% | 46.3% | 285_286ins51;498C>T;781_1632del |
| 3 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_011516926.2 | 44.7% | 44.8% | (many diffs) |
| 4 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_017013218.1 | 44.1% | 40.7% | (many diffs) |
| 5 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_011516927.2 | 43.9% | 42.3% | (many diffs) |
| 6 | human | 225689 | MAPK15 | mitogen-activated protein k... | XM_011516925.2 | 42.4% | 41% | (many diffs) |
| 7 | human | 225689 | MAPK15 | mitogen-activated protein k... | XR_001745493.1 | 39.8% | (many diffs) | |
| 8 | human | 225689 | MAPK15 | mitogen-activated protein k... | XR_001745494.1 | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 897
- ORF length:
- 831
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg caccgtagtg gaccctcgca ttgtccggag atacctactc aggcggcagc 121 tcgggcaggg ggcctatggc attgtgtgga aggcagtgga ccggaggact ggtgaggtcg 181 tggccatcaa gaaaatcttt gatgctttta gggataagac agatgcccag agaacattcc 241 gggaaatcac gctcctccag gagtttgggg accatcccaa catcatcagc ctccttgacg 301 tgatccgggc agagaacgac agggacattt acctggtgtt tgagtttatg ggttgccccc 361 ccagcccccc acccccgact gcagtgcgca ccctctctgc agacactgac ctgaacgcag 421 tcatccggaa gggcggcctg ctgcaggacg tccacgtgcg ctccatcttc taccagctcc 481 tgcgggCCAC CCGGTTCCTC CACTCGGGGC ACGTTGTGCA CCGGGACCAG AAGCCGTCCA 541 ATGTGCTCCT GGATGCCAAC TGCACAGTGA AGCTGTGTGA CTTTGGCCTG GCCCGCTCCC 601 TGGGCGACCT CCCTGAGGGG CCTGAGGACC AGGCCGTGAC AGAGTACGTG GCCACACGCT 661 GGTACCGAGC ACCGGAGGTG CTGCTCTCTT CGCACCGATA CACCCTTGGG GTGGACATGT 721 GGAGTCTGGG CTGTATCCTG GGGGAGATGC TGCGGGGGAG ACCCCTGTTC CCCGGCACGT 781 CCACCCTCCA CCAGCTGGAG CTGATCCTGG AGACCATCCC ACCGCCATCT GAGGAGGACC 841 TCCTGGCTCT CGGCTCAGGC TGCCGTGCCT CTGTGCTGCA CCAGCTGGGG TCCCGGTGCC 901 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 961 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1021 ATATATCTTG TGGAAAGGAC GAAGCTTAAA AACTGCCTTG GATCCAACGC GTTAAGTCga 1081 caatcaacct ctggattaca aaatttgtga aagatt