Construct: ORF TRCN0000466895
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018200.1_s317c1
- Derived from:
- ccsbBroadEn_01788
- DNA Barcode:
- AAGTCCGTTAAAACGGCACTGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YWHAB (7529)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466895
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7529 | YWHAB | tyrosine 3-monooxygenase/tr... | NM_003404.5 | 100% | 100% | |
| 2 | human | 7529 | YWHAB | tyrosine 3-monooxygenase/tr... | NM_139323.4 | 100% | 100% | |
| 3 | human | 7529 | YWHAB | tyrosine 3-monooxygenase/tr... | XM_017028039.2 | 100% | 100% | |
| 4 | human | 7529 | YWHAB | tyrosine 3-monooxygenase/tr... | XM_017028040.1 | 42.4% | 40.8% | (many diffs) |
| 5 | mouse | 54401 | Ywhab | tyrosine 3-monooxygenase/tr... | NM_018753.6 | 90.6% | 98.7% | (many diffs) |
| 6 | mouse | 54401 | Ywhab | tyrosine 3-monooxygenase/tr... | XM_006499909.1 | 90.6% | 98.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 804
- ORF length:
- 738
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac aatggataaa agtgagctgg tacagaaagc caaactcgct gagcaggctg 121 agcgatatga tgatatggct gcagccatga aggcagtcac agaacagggg catgaactct 181 ccaacgaaga gagaaatctg ctctctgttg cctacaagaa tgtggtaggc gcccgccgct 241 cttcctggcg tgtcatctcc agcattgagc agaaaacaga gaggaatgag aagaagcagc 301 agatgggcaa agagtaccgt gagaagatag aggcagaact gcaggacatc tgcaatgatg 361 ttctggagct gttggacaaa tatcttattc ccaatgctac acaaccagaa agtaaggtgt 421 tctacttgaa aatgaaagga gattatttta ggtatctttc tgaagtggca tctggagaca 481 acaaacaaac CACTGTGTCG AACTCCCAGC AGGCTTACCA GGAAGCATTT GAAATTAGTA 541 AGAAAGAAAT GCAGCCTACA CACCCAATTC GTCTTGGTCT GGCACTAAAT TTCTCAGTCT 601 TTTACTATGA GATTCTAAAC TCTCCTGAAA AGGCCTGTAG CCTGGCAAAA ACGGCATTTG 661 ATGAAGCAAT TGCTGAATTG GATACGCTGA ATGAAGAGTC TTATAAAGAC AGCACTCTGA 721 TCATGCAGTT ACTTAGGGAC AATCTCACTC TGTGGACATC GGAAAACCAG GGAGACGAAG 781 GAGACGCTGG GGAGGGAGAG AACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 841 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 901 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA AGTCCGTTAA 961 AACGGCACTG CACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1021 att