Construct: ORF TRCN0000466910
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004294.1_s317c1
- Derived from:
- ccsbBroadEn_04431
- DNA Barcode:
- CCCCCAAGAAGTAACGAAGAGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHF5A (84844)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466910
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84844 | PHF5A | PHD finger protein 5A | NM_032758.4 | 100% | 100% | |
| 2 | mouse | 68479 | Phf5a | PHD finger protein 5A | NM_026737.3 | 91.5% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 396
- ORF length:
- 330
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc taaacatcat ccTGATTTGA TCTTTTGCCG CAAGCAGGCT GGTGTTGCCA 121 TCGGAAGACT GTGTGAAAAA TGTGATGGCA AGTGTGTGAT TTGTGACTCC TATGTGCGTC 181 CCTGCACTCT GGTGCGCATA TGTGATGAGT GTAACTATGG ATCTTACCAG GGGCGCTGTG 241 TGATCTGTGG AGGACCTGGG GTCTCTGATG CCTATTATTG TAAGGAGTGC ACCATCCAGG 301 AGAAGGACAG AGATGGCTGC CCAAAGATTG TCAATCTGGG GAGCTCTAAG ACAGACCTCT 361 TCTATGAACG CAAAAAATAC GGCTTCAAGA AGAGGTGCCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 ACCCCCAAGA AGTAACGAAG AGGGAACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt