Construct: ORF TRCN0000466920

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001130.1_s317c1
Derived from:
ccsbBroadEn_11312
DNA Barcode:
CTTACGAAGTAAATTCTCACCAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NR1I2 (8856)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466920
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8856 NR1I2 nuclear receptor subfamily ... NM_003889.3 87% 87% 1_165del;519_521delGCT
2 human 8856 NR1I2 nuclear receptor subfamily ... NM_022002.2 79.9% 79.9% 1_282del;636_638delGCT
3 human 8856 NR1I2 nuclear receptor subfamily ... NM_033013.2 78.9% 78.9% 1_165del;518_519ins108
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1200
ORF length:
1134
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac atgtgaagga tgcaagggct ttttcaggag ggccatgaaa cgcaacgccc
121ggctgaggtg ccccttccgg aagggcgcct gcgagatcac ccggaagacc cggcgacagt
181gccaggcctg ccgcctgcgc aagtgcctgg agagcggcat gaagaaggag atgatcatgt
241ccgacgaggc cgtggaggag aggcgggcct tgatcaagcg gaagaaaagt gaacggacag
301ggactcagcc actgggagtg caggggctga cagaggagca gcggatgatg atcagggagc
361tgatggacgc tcagatgaaa acctttgaca ctaccttctc ccatttcaag aatttccggc
421caggggtgct tagcagtggc tgcgagttgc cagagtctct gcaggcccca tcgagggaag
481aagctgccaa gtggagccag gtccggaaag atctgtgctc tttgaaggtc tctctgcagc
541tgcgggggga ggatggcagt gtctggaact acaaaccccc agccgacagt ggcgggaaag
601agatcttctc cctgctgccc cacatggctg acatgtcaac ctacatgttc aaaggcatca
661tcagctttgc caaagtcatc tcctacttca gggacttgcc catcgaggac cagatctccc
721tgctgaaggg ggccgctttc gagctgtgtc aactgagatt caacacagtg ttcaacgcgg
781agactggaac ctggGAGTGT GGCCGGCTGT CCTACTGCTT GGAAGACACT GCAGGTGGCT
841TCCAGCAACT TCTACTGGAG CCCATGCTGA AATTCCACTA CATGCTGAAG AAGCTGCAGC
901TGCATGAGGA GGAGTATGTG CTGATGCAGG CCATCTCCCT CTTCTCCCCA GACCGCCCAG
961GTGTGCTGCA GCACCGCGTG GTGGACCAGC TGCAGGAGCA ATTCGCCATT ACTCTGAAGT
1021CCTACATTGA ATGCAATCGG CCCCAGCCTG CTCATAGGTT CTTGTTCCTG AAGATCATGG
1081CTATGCTCAC CGAGCTCCGC AGCATCAATG CTCAGCACAC CCAGCGGCTG CTGCGCATCC
1141AGGACATACA CCCCTTTGCT ACGCCCCTCA TGCAGGAGTT GTTCGGCATC ACAGGTAGCT
1201GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1261GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1321TTTATATATC TTGTGGAAAG GACGACTTAC GAAGTAAATT CTCACCAATA CGCGTTAAGT
1381Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)