Construct: ORF TRCN0000466925
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001017.1_s317c1
- Derived from:
- ccsbBroadEn_05993
- DNA Barcode:
- GGTTCACAGTTTTTGAACTCAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHI3L2 (1117)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466925
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1117 | CHI3L2 | chitinase 3 like 2 | NM_004000.3 | 99.9% | 99.7% | 545C>T |
| 2 | human | 1117 | CHI3L2 | chitinase 3 like 2 | XM_024452752.1 | 99.9% | 99.7% | 545C>T |
| 3 | human | 1117 | CHI3L2 | chitinase 3 like 2 | NM_001025197.1 | 97.3% | 97.1% | 38_39ins30;515C>T |
| 4 | human | 1117 | CHI3L2 | chitinase 3 like 2 | XM_024452753.1 | 97.3% | 97.1% | 38_39ins30;515C>T |
| 5 | human | 1117 | CHI3L2 | chitinase 3 like 2 | XM_024452754.1 | 97.3% | 97.1% | 38_39ins30;515C>T |
| 6 | human | 1117 | CHI3L2 | chitinase 3 like 2 | NM_001025199.2 | 79.6% | 79.4% | 0_1ins237;308C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agcaaccacc atggaccaga agtctctctg ggcaggtgta gtggtcttgc 121 tgcttctcca gggaggatct gcctacaaac tggtttgcta ctttaccaac tggtcccagg 181 accggcagga accaggaaaa ttcacccctg agaatattga ccccttccta tgctctcatc 241 tcatctattc attcgccagc atcgaaaaca acaaggttat catcaaggac aagagtgaag 301 tgatgctcta ccagaccatc aacagtctca aaaccaagaa tcccaaactg aaaattctct 361 tgtccattgg agggtacctg tttggttcca aagggttcca ccctatggtg gattcttcta 421 catcacgctt ggaattcatt aactccataa tcctgtttct gaggaaccat aactttgatg 481 gactggatgt aagctggatc tacccagatc agaaagaaaa cactcatttc actgtgctga 541 ttcatgagtt agcagaagcc tttcagaagg acttcacaaa atccaccaag gaaaggcttc 601 tcttgactgt gggcgtatct gcagggaggc aaatgattga taacagctat caagttgaga 661 aactggcaaa agatctggat ttcatcaacc tcctgtcctt tgacttccat gggtcttggg 721 aaaagcccct tatcactggc cacaacagcc ctctgagcaa ggggtggcag gacagagggc 781 caagctccta ctacaatgtg gaatatgctg tggggtactG GATACATAAG GGAATGCCAT 841 CAGAGAAGGT GGTCATGGGC ATCCCCACAT ATGGGCACTC CTTCACACTG GCCTCTGCAG 901 AAACCACCGT GGGGGCCCCT GCCTCTGGCC CTGGAGCTGC TGGACCCATC ACAGAGTCTT 961 CAGGCTTCCT GGCCTATTAT GAGATCTGCC AGTTCCTGAA AGGAGCCAAG ATCACGCGGC 1021 TCCAGGATCA GCAGGTTCCC TACGCAGTCA AGGGGAACCA GTGGGTGGGC TATGATGATG 1081 TGAAGAGTAT GGAGACCAAG GTTCAGTTCT TAAAGAATTT AAACCTGGGA GGAGCCATGA 1141 TCTGGTCTAT TGACATGGAT GACTTCACTG GCAAATCCTG CAACCAGGGC CCTTACCCTC 1201 TTGTCCAAGC AGTCAAGAGA AGCCTTGGCT CCCTGTGCCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AGGTTCACAG TTTTTGAACT CAACCACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt