Construct: ORF TRCN0000466944
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008583.1_s317c1
- Derived from:
- ccsbBroadEn_02122
- DNA Barcode:
- TATCTTCATCGTTGTCGTTGCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCM2 (9247)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466944
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9247 | GCM2 | glial cells missing transcr... | NM_004752.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1587
- ORF length:
- 1518
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccggcggcc gcggtgcagg aagcggtcgg cgtgtgctcc tacgggatgc 121 agctcagctg ggacatcaac gatccgcaga tgcctcagga gctggccctc tttgaccaat 181 tccgagagtg gcctgacggc tatgtgcgct tcatctacag cagcgatgag aagaaggcac 241 agcgtcacct gagcggctgg gccatgcgca acaccaacaa ccacaatggc cacatcctca 301 agaagtcgtg cctgggtgtg gtggtgtgta cacaggcctg caccctgccc gacggttccc 361 gcctgcagct gaggccggcc atctgcgaca aggcacggct gaaacagcag aagaaggcat 421 gccctaactg tcattctgct ttggagttga ttccttgtcg agggcacagc ggataccccg 481 taaccaactt ttggcggctt gatggcaacg cgatcttttt tcaggccaag ggagttcatg 541 atcatccaag accagagagc aaatcagaga cagaagctag aagaagcgcc atcaagagac 601 aaatggcctc tttctaccaa ccccagaaaa agagaattcg agaatccgag gcagaagaaa 661 atcaagacag cagtggtcat ttcagcaaca tacctccctt ggaaaatcca gaagactttg 721 atatagttac tgaaaccagc ttccctattc cagggcagcc ttgcccttcc ttcccaaagt 781 ctgatgttta caaagctacc tgtgacctag ccacctttca aggagacaaa atgccaccct 841 tccagaaata ctcaagccca agaatctatt tgcctaggcc accttgcagc tatgaattgg 901 caaaccctgg ttatacaaat tcaagcccat atcccaccct ttataaggat tccaccagta 961 tccctaatga cacagactgg gttcatctga acacactaca atgtaatgtc aattcataca 1021 gcagctatga gagaagcttt gatttcacca acaaacagca tggctggaaa ccagctcttg 1081 gaaaacccag ccttgtggaa aggactaacc atgggcagtt tcaggccatg gccactcgcc 1141 cttattataa cccagagctt ccctgcaggt acctcacgac tccaccacca ggtgcccctg 1201 ccctacaaac cgtgatcacc accaccacta aagtgtccta ccaggcctac cagcccccTG 1261 CTATGAAATA CAGTGACAGT GTGCGAGAGG TGAAGAGCCT TTCGAGCTGT AACTATGCTC 1321 CTGAAGATAC TGGGATGTCT GTCTATCCAG AACCCTGGGG TCCTCCGGTG ACAGTCACCA 1381 GGGCAGCCTC TCCTTCAGGG CCACCTCCTA TGAAAATTGC AGGAGATTGC CGGGCCATCA 1441 GACCCACTGT GGCTATTCCC CACGAGCCAG TTTCCTCTAG GACAGATGAA GCAGAGACTT 1501 GGGATGTGTG TCTGTCTGGG CTGGGCTCCG CAGTCAGTTA CTCAGACAGA GTGGGTCCCT 1561 TCTTTACCTA CAACAATGAG GATTTTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1621 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1681 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATATCTTCA 1741 TCGTTGTCGT TGCAGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1801 aagatt