Construct: ORF TRCN0000466985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009812.1_s317c1
- Derived from:
- ccsbBroadEn_11801
- DNA Barcode:
- CATCTGGGATGTCAAGACGTACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHG3 (26030)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | NM_001308147.2 | 99.9% | 100% | 432T>C;729G>A;1791T>C |
| 2 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021157.2 | 99.9% | 100% | 432T>C;729G>A;1791T>C |
| 3 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_024449528.1 | 99.9% | 100% | 432T>C;729G>A;1791T>C |
| 4 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_011536629.3 | 97.5% | 97.5% | (many diffs) |
| 5 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021154.2 | 90.8% | 90.9% | (many diffs) |
| 6 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_011536625.3 | 90.8% | 90.9% | (many diffs) |
| 7 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_011536628.3 | 88.9% | 88.9% | (many diffs) |
| 8 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021155.2 | 88.9% | 88.9% | (many diffs) |
| 9 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021156.2 | 88.9% | 88.9% | (many diffs) |
| 10 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_011536627.3 | 87.5% | 87.6% | (many diffs) |
| 11 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XM_017021158.2 | 74.5% | 74.5% | (many diffs) |
| 12 | human | 26030 | PLEKHG3 | pleckstrin homology and Rho... | XR_002957542.1 | 36.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3726
- ORF length:
- 3657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctgtgtcc acctccctcc accaggatgg cagccaggag cggccggtga 121 gcctgacctc taccacctcc tcgtcgggct cctcctgtga cagtcgcagt gccatggagg 181 agcccagcag ctccgaggct cccgccaaga atggggcagg ctccctgaga agccggcatc 241 tgcccaacag caacaacaac tccagcagct ggttgaacgt gaaggggccc ctctccccgt 301 tcaacagccg ggcagcggca gggcctgcac accacaagct cagctacctg ggccgagtgg 361 tgcgggagat cgtggagaca gagcgcatgt acgtacagga cctgcgcagc atcgtggagg 421 actacctctt gaagatcatt gacacacccg ggctgctgaa gccagaacag gtcagcgccc 481 tctttgggaa catagaaaac atctacgcgc tgaacagcca gctcctcaga gacctggaca 541 gctgcaatag tgaccccgtg gctgtggcca gctgctttgt ggaaaggagc caagagtttg 601 atatctacac tcagtattgc aacaattacc ccaactccgt ggccgccctg acggaatgca 661 tgcgggacaa gcagcaggcc aagttctttc gggaccggca ggagctgcta cagcactcgc 721 tgcccttggg ctcctacctg ctgaagccag tccagcgcat cctcaagtac cacctgctgc 781 tccaggaaat tgccaaacat tttgatgaag aagaggatgg ctttgaggtg gtggaggatg 841 ccattgacac catgacctgt gtggcctggt acatcaacga catgaagagg aggcatgagc 901 acgcggtccg gctccaggag attcagtcac tcctcatcaa ctggaagggg cccgacctga 961 ccacctacgg ggagcttgtc ctggagggca cattccgcgt gcatcgcgtg cgcaatgaaa 1021 ggaccttttt cctctttgac aaaacactgc ttatcaccaa gaagcggggc gatcactttg 1081 tctacaaggg caacatcccg tgctcctccc tgatgctgat cgaaagcacc agagactccc 1141 tgtgcttcac tgtcacccac tacaagcaca gcaagcagca gtacagcatc caggccaaga 1201 cagtggagga gaaacggaac tggactcacc acatcaagag gctcatccta gagaaccacc 1261 atgccaccat tccccagaag gccaaggaag ccatcttgga aatggattcc tattatccca 1321 atcggtaccg ctgcagccca gagcggctga agaaggcttg gtcctcccag gatgaggtgt 1381 ccaccaatgt gcgccagggg cgccggcaat ctgagccaac caaacacctg ctcaggcaac 1441 tcaacgagaa agcccgagca gcaggaatga agggaaaggg gcgcagggag tctgaaagct 1501 ccaggagcag cagaaggccc agtggccggt ctccaaccag tactgagaag cgcatgagct 1561 tcgagtccat ttcttccctg ccagaggttg agccggaccc tgaggctggg agtgagcaag 1621 aggtattttc tgctgtggaa gggcccagtg ccgaggagac gccttcagac acagaatctc 1681 cagaagtcct ggagacacag cttgatgccc accagggcct tctggggatg gaccccccag 1741 gtgacatggt ggacttcgtg gcagctgaga gcactgagga ccttaaggcc ctgagcagcg 1801 aggaggaaga agaaatggga ggtgccgccc aggagcctga gagccttctg ccaccctccg 1861 tgctggacca ggccagcgtc attgcggagc gatttgtcag cagcttctct cggcggagca 1921 gcgtggcaca ggaggacagc aagtccagtg gctttgggag cccgcggctg gtcagccgga 1981 gcagcagcgt gctcagcctg gagggcagcg agaagggcct ggcccggcat ggcagtgcca 2041 cagactccct cagctgtcag ctctccccag aagtggacat cagtgtgggg gtggccacag 2101 aggacagccc ttctgtcaat gggatggagc ccccaagccc aggctgccca gtggagcctg 2161 accggtcttc ctgcaagaag aaggaatcag cactctccac ccgagaccgg ctgttgctag 2221 acaagattaa gagctattat gaaaatgcag aacaccatga tgcaggcttc agcgtccgtc 2281 gccgggagag cctctcctac atccccaaag gactggtaag aaactccatc tccaggttca 2341 acagccttcc ccggccagac ccagagccag tacctccagt ggggagcaag agacaggtgg 2401 gctcccggcc gacttcgtgg gccctgtttg agctcccagg accaagccag gcagtcaaag 2461 gggacccacc tcccatctca gatgctgagt tccgcccatc ttcagaaatt gtgaagatct 2521 gggagggaat ggagtcttcc ggagggagcc ctgggaaggg gccaggccag ggccaggcca 2581 atggctttga cctgcatgag ccactcttca tcctggagga gcatgagctg ggagccatca 2641 cagaggagtc ggccactgcc tccccggaaa gctcctctcc cactgagggg cgcagcccgg 2701 cccacctggc ccgggagctg aaagagctgg tgaaggagct gagcagcagt acccaggggg 2761 agctggtggc cccactgcac ccccgcatcg tgcagctctc ccacgtaatg gacagccacg 2821 tgagcgagcg cgtcaagaac aaggtctacc agctggcccg ccagtacagc ctccggatca 2881 agagcaacaa gccagtgatg gccaggccac cactgcagtg ggaaaaggtg gcccctgaga 2941 gggatgggaa gagccccact gtgccctgtc tacaggaaga ggctggagag ccattaggtg 3001 gcaaaggtaa gaggaagccg gtgctgtctc tatttgacta tgagcagctg atggcccagg 3061 agcacagccc tcccaagccc tcctcggctg gggagatgtc accacagcgt ttcttcttca 3121 acccgtctgc tgtcagccag aggaccacct cgcctggggg ccggccctcc gcccggagcc 3181 ccctcagccc cacagagacc ttcagctggc ccgacgtccg tgagctctgc tccaagtatg 3241 cctcccgcga tgaggcacgc cgagcagggg gcggccggcc ccgcggccca cccgtcaaca 3301 ggagccactc ggtgccggag aacatggtag agccacctct gtcgggcagg gtgggccgct 3361 gccgcagcct gagcaccaag aggggccggg gaggcggaga ggctgcccaa tcccctgggc 3421 cTCTGCCCCA GAGCAAGCCG GATGGAGGCG AGACCCTGTA TGTCACTGCA GACCTCACCC 3481 TGGAGGACAA CCGGCGGGTG ATTGTCATGG AGAAGGGACC CCTTCCCAGC CCCACTGCAG 3541 GGCTGGAGGA GAGCAGTGGC CAGGGACCAA GCTCACCGGT GGCCCTGCTG GGGCAGGTTC 3601 AGGACTTCCA GCAGTCTGCA GAGTGCCAGC CGAAGGAAGA GGGTTCCAGG GACCCGGCAG 3661 ACCCGAGCCA GCAGGGCAGA GTGAGAAACC TTAGAGAGAA GTTCCAGGCC TTGAACTCTG 3721 TCGGTTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 3781 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3841 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACATCTGGGA TGTCAAGACG TACAAACGCG 3901 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt