Construct: ORF TRCN0000467000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006572.1_s317c1
- Derived from:
- ccsbBroadEn_03162
- DNA Barcode:
- GAGTCGCTCCAGCTTCTTACAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBX22 (50945)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50945 | TBX22 | T-box transcription factor 22 | NM_001109878.2 | 100% | 100% | |
| 2 | human | 50945 | TBX22 | T-box transcription factor 22 | NM_016954.2 | 100% | 100% | |
| 3 | human | 50945 | TBX22 | T-box transcription factor 22 | NM_001109879.2 | 76.9% | 76.9% | 0_1ins360 |
| 4 | human | 50945 | TBX22 | T-box transcription factor 22 | NM_001303475.1 | 76.9% | 76.9% | 0_1ins360 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1626
- ORF length:
- 1560
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctgagctct cgggcgcgtg ccttctccgt ggaagccttg gtggggagac 121 ccagcaaaag aaaactccaa gacccaatac aggcggagca gcctgagctg cgggagaaaa 181 agggcggaga ggaagaggag gagagaagga gcagcgctgc agggaagagc gagccgcttg 241 aaaaacaacc taagacagag ccctcaacat ctgcttcctc tggctgcggc agcgacagcg 301 gctacggcaa cagctctgaa agtctggaag agaaagatat tcaaatggag cttcaaggat 361 ctgaactgtg gaaaagattc catgacatcg ggactgagat gatcattact aaagcgggca 421 ggcggatgtt cccctctgtt cgggtcaagg tgaaagggtt ggatccaggg aagcagtacc 481 atgtggccat cgatgtggtg ccggtggatt ccaaacgcta taggtacgtc tatcacagct 541 cacagtggat ggtagctggg aatacagacc atttgtgcat cattcctaga ttctatgttc 601 acccggactc accctgctcg ggagagacct ggatgcggca gatcatcagc tttgatcgca 661 tgaaactcac caacaatgag atggatgaca aaggccacat cattctgcaa tccatgcata 721 agtacaaacc ccgagtgcac gtgatagagc aaggcagcag tgttgacctg tcccagattc 781 agtccttgcc cactgaaggt gttaaaacat tctcctttaa agaaactgag ttcaccacag 841 taacggctta ccaaaaccaa cagattacga aactaaaaat agaaagaaat ccttttgcta 901 aaggatttag agatactgga agaaacaggg gtgtattgga tgggctttta gagacctacc 961 catggaggcc ttctttcact ctcgatttta aaacctttgg cgcagacaca caaagtggaa 1021 gcagtggctc atctccagtg acctctagtg gaggggcccc ctctcctttg aactccttac 1081 tttctccact ttgcttttca cctatgtttc atttacctac aagctccctt ggaatgccct 1141 gtccagaggc atacctgccc aatgtcaacc tgcctctatg ctacaagatt tgtccaacta 1201 atttttggca acagcaacct cttgttttac cggctccTGA AAGACTAGCA AGCAGCAACA 1261 GTTCTCAGTC TTTAGCCCCA CTCATGATGG AAGTGCCTAT GTTATCTTCC CTGGGGGTCA 1321 CCAATTCAAA AAGCGGTTCA TCTGAAGACT CCAGTGATCA GTATCTACAA GCACCTAATT 1381 CTACCAATCA AATGTTATAT GGATTACAGT CACCTGGAAA TATTTTTCTG CCAAACTCCA 1441 TCACCCCAGA AGCACTTAGT TGCTCCTTTC ATCCTTCCTA TGACTTTTAT AGATACAATT 1501 TCTCTATGCC ATCTAGACTG ATAAGTGGTT CCAACCATCT TAAAGTGAAT GACGACAGTC 1561 AAGTTTCTTT TGGAGAAGGC AAATGTAATC ATGTTCATTG GTATCCAGCA ATTAACCATT 1621 ACCTTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1681 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1741 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGAGTCGCTC CAGCTTCTTA CAACAACGCG 1801 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt