Construct: ORF TRCN0000467007
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019042.1_s317c1
- Derived from:
- ccsbBroadEn_14597
- DNA Barcode:
- TACTGCGCTTTGTGACAAACGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSF1R (1436)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467007
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1436 | CSF1R | colony stimulating factor 1... | NM_001288705.2 | 100% | 100% | |
| 2 | human | 1436 | CSF1R | colony stimulating factor 1... | NM_001349736.1 | 100% | 100% | |
| 3 | human | 1436 | CSF1R | colony stimulating factor 1... | NM_005211.3 | 100% | 100% | |
| 4 | human | 1436 | CSF1R | colony stimulating factor 1... | NR_109969.1 | 70.1% | 1_213del;2180_2181ins163;2967_3760del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2985
- ORF length:
- 2916
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcccagga gttctgctgc tcctgctggt ggccacagct tggcatggtc 121 agggaatccc agtgatagag cccagtgtcc ctgagctggt cgtgaagcca ggagcaacgg 181 tgaccttgcg atgtgtgggc aatggcagcg tggaatggga tggcccccca tcacctcact 241 ggaccctgta ctctgatggc tccagcagca tcctcagcac caacaacgct accttccaaa 301 acacggggac ctatcgctgc actgagcctg gagaccccct gggaggcagc gccgccatcc 361 acctctatgt caaagaccct gcccggccct ggaacgtgct agcacaggag gtggtcgtgt 421 tcgaggacca ggacgcacta ctgccctgtc tgctcacaga cccggtgctg gaagcaggcg 481 tctcgctggt gcgtgtgcgt ggccggcccc tcatgcgcca caccaactac tccttctcgc 541 cctggcatgg cttcaccatc cacagggcca agttcattca gagccaggac tatcaatgca 601 gtgccctgat gggtggcagg aaggtgatgt ccatcagcat ccggctgaaa gtgcagaaag 661 tcatcccagg gcccccagcc ttgacactgg tgcctgcaga gctggtgcgg attcgagggg 721 aggctgccca gatcgtgtgc tcagccagca gcgttgatgt taactttgat gtcttcctcc 781 aacacaacaa caccaagctc gcaatccctc aacaatctga ctttcataat aaccgttacc 841 aaaaagtcct gaccctcaac ctcgatcaag tagatttcca acatgccggc aactactcct 901 gcgtggccag caacgtgcag ggcaagcact ccacctccat gttcttccgg gtggtagaga 961 gtgcctactt gaacttgagc tctgagcaga acctcatcca ggaggtgacc gtgggggagg 1021 ggctcaacct caaagtcatg gtggaggcct acccaggcct gcaaggtttt aactggacct 1081 acctgggacc cttttctgac caccagcctg agcccaagct tgctaatgct accaccaagg 1141 acacatacag gcacaccttc accctctctc tgccccgcct gaagccctct gaggctggcc 1201 gctactcctt cctggccaga aacccaggag gctggagagc tctgacgttt gagctcaccc 1261 ttcgataccc cccagaggta agcgtcatat ggacattcat caacggctct ggcacccttt 1321 tgtgtgctgc ctctgggtac ccccagccca acgtgacatg gctgcagtgc agtggccaca 1381 ctgataggtg tgatgaggcc caagtgctgc aggtctggga tgacccatac cctgaggtcc 1441 tgagccagga gcccttccac aaggtgacgg tgcagagcct gctgactgtt gagaccttag 1501 agcacaacca aacctacgag tgcagggccc acaacagcgt ggggagtggc tcctgggcct 1561 tcatacccat ctctgcagga gcccacacgc atcccccgga tgagttcctc ttcacaccag 1621 tggtggtcgc ctgcatgtcc atcatggcct tgctgctgct gctgctcctg ctgctattgt 1681 acaagtataa gcagaagccc aagtaccagg tccgctggaa gatcatcgag agctatgagg 1741 gcaacagtta tactttcatc gaccccacgc agctgcctta caacgagaag tgggagttcc 1801 cccggaacaa cctgcagttt ggtaagaccc tcggagctgg agcctttggg aaggtggtgg 1861 aggccacggc ctttggtctg ggcaaggagg atgctgtcct gaaggtggct gtgaagatgc 1921 tgaagtccac ggcccatgct gatgagaagg aggccctcat gtccgagctg aagatcatga 1981 gccacctggg ccagcacgag aacatcgtca accttctggg agcctgtacc catggaggcc 2041 ctgtactggt catcacggag tactgttgct atggcgacct gctcaacttt ctgcgaagga 2101 aggctgaggc catgctggga cccagcctga gccccggcca ggaccccgag ggaggcgtcg 2161 actataagaa catccacctc gagaagaaat atgtccgcag ggacagtggc ttctccagcc 2221 agggtgtgga cacctatgtg gagatgaggc ctgtctccac ttcttcaaat gactccttct 2281 ctgagcaaga cctggacaag gaggatggac ggcccctgga gctccgggac ctgcttcact 2341 tctccagcca agtagcccag ggcatggcct tcctcgcttc caagaattgc atccaccggg 2401 acgtggcagc gcgtaacgtg ctgttgacca atggtcatgt ggccaagatt ggggacttcg 2461 ggctggctag ggacatcatg aatgactcca actacattgt caagggcaat gcccgcctgc 2521 ctgtgaagtg gatggcccca gagagcatct ttgactgtgt ctacacggtt cagagcgacg 2581 tctggtccta tggcatcctc ctctgggaga tcttctcact tgggctgaat ccctaccctg 2641 gcatccTGGT GAACAGCAAG TTCTATAAAC TGGTGAAGGA TGGATACCAA ATGGCCCAGC 2701 CTGCATTTGC CCCAAAGAAT ATATACAGCA TCATGCAGGC CTGCTGGGCC TTGGAGCCCA 2761 CCCACAGACC CACCTTCCAG CAGATCTGCT CCTTCCTTCA GGAGCAGGCC CAAGAGGACA 2821 GGAGAGAGCG GGACTATACC AATCTGCCGA GCAGCAGCAG AAGCGGTGGC AGCGGCAGCA 2881 GCAGCAGTGA GCTGGAGGAG GAGAGCTCTA GTGAGCACCT GACCTGCTGC GAGCAAGGGG 2941 ATATCGCCCA GCCCTTGCTG CAGCCCAACA ACTATCAGTT CTGCTTGCCA ACTTTCTTGT 3001 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3061 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3121 GAAAGGACGA TACTGCGCTT TGTGACAAAC GGTTACGCGT TAAGTCgaca atcaacctct 3181 ggattacaaa atttgtgaaa gatt