Construct: ORF TRCN0000467009
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002045.2_s317c1
- Derived from:
- ccsbBroadEn_15087
- DNA Barcode:
- ATACCCTGTCGGTTGGTCAATCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ULK4 (54986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467009
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54986 | ULK4 | unc-51 like kinase 4 | NM_001322500.2 | 46.4% | 45% | (many diffs) |
| 2 | human | 54986 | ULK4 | unc-51 like kinase 4 | NM_017886.4 | 44.9% | 43.5% | (many diffs) |
| 3 | human | 54986 | ULK4 | unc-51 like kinase 4 | NR_136342.2 | 39.2% | (many diffs) | |
| 4 | human | 54986 | ULK4 | unc-51 like kinase 4 | NM_001322501.2 | 21.2% | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1806
- ORF length:
- 1740
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaactttatt ctgtatgagg agatcggaag aggaagcaag actgttgtct 121 ataaagggcg acggaaggga acaatcaatt ttgtagccat tctttgtact gataagtgca 181 aaaggcctga aataaccaac tgggtccgtc tcacccgtga aataaaacac aagaatattg 241 taacttttca tgaatggtat gaaacaagca accacctctg gctagtggtg gaactctgca 301 caggtggttc cttaaaaaca gttattgctc aagatgaaaa cctcccagaa gatgttgtga 361 gagaatttgg aattgacctg attagtggat tacatcatct tcataaactt ggcattctct 421 tttgtgacat ttctcctagg aagatactct tggaagggcc tggcacactg aagtttagca 481 acttttgctt ggcaaaagtg gaaggtgaaa atttggaaga gttctttgct ttggtggcag 541 cagaggaagg aggaggtgat aatggggaaa atgtcctgaa gaaaagcatg aaaagtagag 601 tcaaaggatc tcctgtatat acagcaccag aagttgtgag gggtgctgac ttttccatct 661 ccagtgacct ctggtctttg ggctgtctgc tttatgaaat gttttcagga aaacctccat 721 tcttctcaga aagtatttca gaattaactg aaaagatctt atgtgaagat cctttgccac 781 ctattccgaa agattcttct cgtcctaaag cttcttcaga ttttattaat ttgcttgatg 841 ggttacttca aagagatcct cagaaaagat tgacttggac aaggctactg cagcattcat 901 tttggaagaa agcttttgct ggagcagatc aggaatcaag cgtcgaagat ctcagtctca 961 gcagaaacac tatggagtgt tctgggccac aagattccaa ggagcttttg cagaactctc 1021 agagtagaca agcaaaaggg cacaagagtg gtcaaccact aggtcactct ttcagactag 1081 aaaatccaac tgagtttcgg cctaagagta ctcttgaggg tcaattgaat gaatccatgt 1141 ttcttctcag ttctcgtcct actcccagaa ctagcactgc agtggaagta agtcctggtg 1201 aggatatgac tcactgttca ccacagaaga cttctcctct gaccaagatt acaagtggac 1261 acctgagtca gcaggacctg gaatcccaga tgagagagct tatctacacg gactcagatc 1321 ttgttgtcac ccccattatc gacaatccaa agataatgaa acagccacca gttaaatttg 1381 atgcaaaaat attgcatcta ccaacatatt cagtggataa gttattattt ctgaaagatc 1441 aagattggaa tgacttttTG CAACAAGTGT GCTCGCAGAT CGACTCCACT GAGAAGAGCA 1501 TGGGGGCCTC CCGAGCCAAG CTGAATCTCC TTTGCTATTT GTGCGTGGTG GCTGGTCACC 1561 AGGAGGTGGC CACCAGGCTC CTCCATTCCC CCCTGTTCCA ATTGCTAATC CAGCATTTGC 1621 GGATAGCTCC AAACTGGGAT ATACGGGCCA AGGTTGCTCA CGTAATTGGT TTACTGGCTT 1681 CGCACACAGC TGAGCTCCAG GAAAATACAC CTGTTGTTGA GACTACAAGC TCCATTGGAA 1741 TCGGGATTTT GAACTGTCTT GTTCAACACT CCACTCCAGT GCCTAGACAG TGCCTTGTGT 1801 ATGTATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1861 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1921 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATACCCTGT CGGTTGGTCA ATCACACGCG 1981 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt