Construct: ORF TRCN0000467013
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010720.1_s317c1
- Derived from:
- ccsbBroadEn_15349
- DNA Barcode:
- TAAAAGTATTAAACATGTTCTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467013
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 317772 | H2AC21 | H2A clustered histone 21 | NM_175065.2 | 93.5% | 89.2% | (many diffs) |
| 2 | human | 8338 | H2AC20 | H2A clustered histone 20 | NM_003517.2 | 82.6% | 85.3% | (many diffs) |
| 3 | mouse | 621893 | Hist2h2ab | histone cluster 2, H2ab | NM_178213.4 | 83.1% | 89.2% | (many diffs) |
| 4 | mouse | 319176 | Hist2h2ac | histone cluster 2, H2ac | NM_175662.2 | 81.2% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 459
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcaggacgc ggaaagcagg gaggcaaggc ccgcgccaag gccaagtcgc 121 gctcgtcccg cgccggtccc cagcccccgg cggggcgagc gcaccgcccG CCGCGCAAAG 181 GCAACCACGC GGAGCGGGTC GGGGCAGGCG CCCCGGTGTA CCCGGCGGCG GTCCTCGAGC 241 ACCTGACCGC GGAAACTCCG GAGCCGGCGG GCAACGCGGC TCGGGACAAC AAGAAGACGC 301 GCATCATCCC TCGCCATCTG CAACTAGCCG TGAGAAACGA CGAAGAGCTC AACAAGCTAC 361 TCGGGGGTGT CACCATTGCC CAGGGCGGCG TCCCGCCTAA CATCCAGGCC GTCCTGTCGC 421 CCAAGAAAAC GGAGAGTCAC AAGCCTGGCA AGAACAAGTT GCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGATAAAAG TATTAAACAT GTTCTTATAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt