Transcript: Mouse NM_178213.4

Mus musculus histone cluster 2, H2ab (Hist2h2ab), mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Hist2h2ab (621893)
Length:
438
CDS:
1..393

Additional Resources:

NCBI RefSeq record:
NM_178213.4
NBCI Gene record:
Hist2h2ab (621893)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_178213.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000255258 CAAGGCGAAGTCGCGGTCGTC pLKO_005 39 CDS 100% 0.000 0.000 N Hist2h2ab n/a
2 TRCN0000074588 CAACAAGAAGACGCGCATCAT pLKO.1 219 CDS 100% 4.950 2.475 Y H2AC18 n/a
3 TRCN0000242129 CAACAAGAAGACGCGCATCAT pLKO_005 219 CDS 100% 4.950 2.475 Y Hist1h2ad n/a
4 TRCN0000097001 ACAACAAGAAGACGCGCATCA pLKO.1 218 CDS 100% 4.050 2.025 Y Hist2h2ac n/a
5 TRCN0000256521 ACAACAAGAAGACGCGCATCA pLKO_005 218 CDS 100% 4.050 2.025 Y H2AC19 n/a
6 TRCN0000257783 ACAAGAAGACGCGCATCATCC pLKO_005 221 CDS 100% 4.050 2.025 Y Hist1h2an n/a
7 TRCN0000429705 ACAAGAAGACGCGCATCATCC pLKO_005 221 CDS 100% 4.050 2.025 Y H2AC20 n/a
8 TRCN0000257997 GACAACAAGAAGACGCGCATC pLKO_005 217 CDS 100% 2.250 1.125 Y Hist1h2af n/a
9 TRCN0000092689 CGACAACAAGAAGACGCGCAT pLKO.1 216 CDS 100% 2.160 1.080 Y Hist2h2aa2 n/a
10 TRCN0000429526 CGACAACAAGAAGACGCGCAT pLKO_005 216 CDS 100% 2.160 1.080 Y H2AW n/a
11 TRCN0000255256 GGAGTACCTAACGGCCGAGAT pLKO_005 168 CDS 100% 1.350 0.675 Y Hist2h2ab n/a
12 TRCN0000255257 CAAGAAGACGCGCATCATCCC pLKO_005 222 CDS 100% 0.720 0.360 Y Hist2h2ab n/a
13 TRCN0000097003 TGGAGTACCTAACGGCCGAGA pLKO.1 167 CDS 100% 0.720 0.360 Y Hist2h2ac n/a
14 TRCN0000092692 GCGACAACAAGAAGACGCGCA pLKO.1 215 CDS 100% 0.180 0.090 Y Hist2h2aa2 n/a
15 TRCN0000256452 ACGGCCGAGATCCTGGAGCTG pLKO_005 178 CDS 100% 0.000 0.000 Y Hist1h2ai n/a
16 TRCN0000255946 CCCGCGACAACAAGAAGACGC pLKO_005 212 CDS 100% 0.000 0.000 Y Hist1h2ah n/a
17 TRCN0000092690 CGGCGGTGCTGGAGTACCTAA pLKO.1 158 CDS 100% 0.000 0.000 Y Hist2h2aa2 n/a
18 TRCN0000097002 GCGCAAGGGCAACTACGCGGA pLKO.1 105 CDS 100% 0.000 0.000 Y Hist2h2ac n/a
19 TRCN0000092691 GCTGCTGCGCAAGGGCAACTA pLKO.1 99 CDS 100% 0.000 0.000 Y Hist2h2aa2 n/a
20 TRCN0000097000 GCTGGAGTACCTAACGGCCGA pLKO.1 165 CDS 100% 0.000 0.000 Y Hist2h2ac n/a
21 TRCN0000255259 TGCTGCGCAAGGGCAACTACG pLKO_005 101 CDS 100% 0.000 0.000 Y Hist2h2ab n/a
22 TRCN0000267543 TGTGCACCGGCTGCTGCGCAA pLKO_005 90 CDS 100% 0.000 0.000 Y Hist2h2ab n/a
23 TRCN0000106666 GCCGAGATCCTGGAGCTGGCT pLKO.1 181 CDS 100% 0.000 0.000 Y H2AC13 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178213.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01896 pDONR223 100% 83.3% 96.1% None (many diffs) n/a
2 ccsbBroad304_01896 pLX_304 0% 83.3% 96.1% V5 (many diffs) n/a
3 TRCN0000480994 TCCGAGTATGCGTATGATAAGCCC pLX_317 94.3% 83.3% 96.1% V5 (many diffs) n/a
4 ccsbBroadEn_15349 pDONR223 96.6% 83.1% 89.2% None (many diffs) n/a
5 ccsbBroad304_15349 pLX_304 0% 83.1% 89.2% V5 (many diffs) n/a
6 TRCN0000467013 TAAAAGTATTAAACATGTTCTTAT pLX_317 72.6% 83.1% 89.2% V5 (many diffs) n/a
7 ccsbBroadEn_15630 pDONR223 0% 82.1% 95.3% None (many diffs) n/a
8 ccsbBroad304_15630 pLX_304 0% 82.1% 95.3% V5 (many diffs) n/a
9 TRCN0000466536 CGATTGTCATTCGGCCGACTATTC pLX_317 51.9% 82.1% 95.3% V5 (many diffs) n/a
10 TRCN0000479809 GGTCGAATGGTCGCATTAGATTCC pLX_317 80.6% 81.6% 95.3% V5 (many diffs) n/a
11 ccsbBroadEn_15631 pDONR223 0% 81.4% 95.3% None (many diffs) n/a
12 ccsbBroad304_15631 pLX_304 0% 81.4% 95.3% V5 (many diffs) n/a
13 ccsbBroadEn_11266 pDONR223 100% 81.4% 95.3% None (many diffs) n/a
14 ccsbBroad304_11266 pLX_304 0% 81.4% 95.3% V5 (many diffs) n/a
15 TRCN0000473665 CTTGAACTGGACTCCCACCGCACC pLX_317 98.5% 81.4% 95.3% V5 (many diffs) n/a
16 ccsbBroadEn_01893 pDONR223 100% 81.2% 93.8% None (many diffs) n/a
17 ccsbBroad304_01893 pLX_304 97.8% 81.2% 93.8% V5 (many diffs) n/a
18 TRCN0000469277 CTTGTACACGCATGGACCTTCTTT pLX_317 100% 81.2% 93.8% V5 (many diffs) n/a
19 ccsbBroadEn_04591 pDONR223 100% 81.2% 93.8% None (many diffs) n/a
20 ccsbBroad304_04591 pLX_304 0% 81.2% 93.8% V5 (many diffs) n/a
21 TRCN0000472223 CCTTGCTACGATGTATGAGACTTA pLX_317 100% 81.2% 93.8% V5 (many diffs) n/a
22 ccsbBroadEn_01892 pDONR223 100% 80.4% 93.8% None (many diffs) n/a
23 ccsbBroad304_01892 pLX_304 0% 80.4% 93.8% V5 (many diffs) n/a
24 TRCN0000473552 GACAAACTTCCATCCTCTTGAACC pLX_317 98.5% 80.4% 93.8% V5 (many diffs) n/a
25 ccsbBroadEn_02055 pDONR223 100% 80.3% 93.8% None (many diffs) n/a
26 ccsbBroad304_02055 pLX_304 0% 80.3% 93.8% V5 (many diffs) n/a
27 TRCN0000466333 TGGGGTTTAATTGCATTCTTGTTA pLX_317 72.6% 80.3% 93.8% V5 (many diffs) n/a
28 ccsbBroadEn_01894 pDONR223 100% 80.2% 94.6% None (many diffs) n/a
29 ccsbBroad304_01894 pLX_304 0% 80.2% 94.6% V5 (many diffs) n/a
30 TRCN0000466135 ATCGATAACCATCGCTTACCTGGA pLX_317 72.6% 80.2% 94.6% V5 (many diffs) n/a
31 ccsbBroadEn_03650 pDONR223 100% 80% 93.8% None (many diffs) n/a
32 ccsbBroad304_03650 pLX_304 0% 80% 93.8% V5 (many diffs) n/a
33 TRCN0000478286 ACAGCCCAAGTATGTTGCTGTGAT pLX_317 83.7% 80% 93.8% V5 (many diffs) n/a
34 ccsbBroadEn_15629 pDONR223 0% 80% 94.6% None (many diffs) n/a
35 ccsbBroad304_15629 pLX_304 0% 80% 94.6% V5 (many diffs) n/a
36 TRCN0000469353 AGACAGTAAACCCGGGATGCGCAC pLX_317 73.4% 80% 94.6% V5 (many diffs) n/a
37 ccsbBroadEn_15628 pDONR223 0% 79.8% 93% None (many diffs) n/a
38 ccsbBroad304_15628 pLX_304 0% 79.8% 93% V5 (many diffs) n/a
39 TRCN0000474646 TCTACAGTCTCGTAGCTATGTTCA pLX_317 100% 79.8% 93% V5 (many diffs) n/a
40 ccsbBroadEn_01891 pDONR223 100% 79.7% 93.8% None (many diffs) n/a
41 ccsbBroad304_01891 pLX_304 0% 79.7% 93.8% V5 (many diffs) n/a
42 TRCN0000481190 CGTGTTGGCCGTTCGATTTAGAAT pLX_317 98.5% 79.7% 93.8% V5 (many diffs) n/a
43 ccsbBroadEn_07222 pDONR223 100% 79.6% 93% None (many diffs) n/a
44 ccsbBroad304_07222 pLX_304 0% 79.6% 93% V5 (many diffs) n/a
45 TRCN0000474296 CCGAGAACTTAGACACGTTCATCC pLX_317 98.5% 79.6% 93% V5 (many diffs) n/a
46 ccsbBroadEn_09253 pDONR223 100% 78.6% 93% None (many diffs) n/a
47 ccsbBroad304_09253 pLX_304 0% 78.6% 93% V5 (many diffs) n/a
48 ccsbBroadEn_01895 pDONR223 100% 78.1% 93.8% None (many diffs) n/a
49 ccsbBroad304_01895 pLX_304 0% 78.1% 93.8% V5 (many diffs) n/a
50 TRCN0000468567 AACCATAGCTAACCCTGCACACAC pLX_317 100% 78.1% 93.8% V5 (many diffs) n/a
51 ccsbBroadEn_00717 pDONR223 100% 75.5% 83.2% None (many diffs) n/a
52 ccsbBroad304_00717 pLX_304 0% 75.5% 83.2% V5 (many diffs) n/a
53 TRCN0000469289 TCCAAGCCCTCATTGGAGCTGGTC pLX_317 100% 75.5% 83.2% V5 (many diffs) n/a
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