Construct: ORF TRCN0000467020
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001351.1_s317c1
- Derived from:
- ccsbBroadEn_09172
- DNA Barcode:
- TTCATTTTCCATGGAAACCTATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A33 (84275)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467020
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84275 | SLC25A33 | solute carrier family 25 me... | NM_032315.3 | 99.8% | 99.6% | 320T>C |
| 2 | human | 84275 | SLC25A33 | solute carrier family 25 me... | XM_011542296.1 | 80.2% | 80% | 0_1ins189;131T>C |
| 3 | human | 84275 | SLC25A33 | solute carrier family 25 me... | XM_005263503.4 | 76.8% | 73.7% | (many diffs) |
| 4 | mouse | 70556 | Slc25a33 | solute carrier family 25, m... | NM_027460.2 | 86% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1029
- ORF length:
- 963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacgggcggc cagcagaagg agaacacgct gcttcacctc ttcgccggcg 121 ggtgtggagg cacagttggt gctattttca cttgtccact agaagtcatt aagacacggt 181 tgcagtcttc aagattagct ctccggacag tctactatcc tcaggttcat ctggggacca 241 ttagtggagc tggaatggtg agaccaacat ccgtgacacc tggactcttt caggttctga 301 agtcgatctt ggagaaagag ggaccaaagt cactttttag aggcttgggt ccaaatttgg 361 ttggagttgc accatcaagg gctgcatact ttgcatgtta ctccaaagcc aaagagcaat 421 ttaatggcat tttcgtgcct aacagcaata ttgtgcatat tttctcagct ggctctgcag 481 cttttatcac aaattcctta atgaatccta tatggatggt taaaacccga atgcagctag 541 aacagaaagt gaggggctct aagcagatga atacactcca gtgtgctcgt tacgtttacc 601 agaccgaagg cattcgtggc ttctatagag gattaactgc ctcgtatgct ggaatttccg 661 aaactataat ctgcttTGCT ATTTATGAAA GTTTAAAGAA GTATCTGAAA GAAGCTCCAT 721 TAGCCTCTTC TGCAAATGGG ACTGAGAAAA ATTCCACAAG TTTTTTTGGA CTTATGGCAG 781 CTGCTGCTCT TTCTAAGGGC TGTGCCTCCT GCATTGCTTA TCCACACGAA GTCATAAGGA 841 CGAGGCTCCG GGAAGAGGGC ACCAAGTACA AGTCTTTTGT CCAGACGGCG CGCCTGGTGT 901 TCCGGGAAGA AGGCTACCTT GCCTTTTATA GAGGACTGTT TGCCCAGCTT ATCCGGCAGA 961 TCCCAAATAC TGCCATTGTG TTGTCTACTT ATGAGTTAAT TGTGTACCTG TTAGAAGACC 1021 GTACTCAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTCATT TTCCATGGAA ACCTATGTAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt