Construct: ORF TRCN0000467033
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001112.1_s317c1
- Derived from:
- ccsbBroadEn_11687
- DNA Barcode:
- TATCTGGGCGCTCCACACCTACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABRAXAS2 (23172)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467033
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23172 | ABRAXAS2 | abraxas 2, BRISC complex su... | XM_011539554.1 | 100% | 100% | |
| 2 | human | 23172 | ABRAXAS2 | abraxas 2, BRISC complex su... | XM_017015986.1 | 100% | 100% | |
| 3 | human | 23172 | ABRAXAS2 | abraxas 2, BRISC complex su... | NM_032182.4 | 74.9% | 74.9% | 1_312del |
| 4 | mouse | 109359 | Abraxas2 | BRISC complex subunit | NM_198017.3 | 66% | 67.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 999
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaag 61 ttggcatgtc ctacagagag caggttcttc acaagcagct cacccgcatc ctcggcgtgc 121 ccgacctcgt ctttcttctc ttcagcttca tctccactgc caacaattcc actcacgctt 181 tagaatatgt gctcttcaga ccaaatagaa ggtataatca gaggatatca ctcgctattc 241 ccaatctagg aaatactagc cagcaagagt acaaagtgtc ttcagtgcca aatacttctc 301 agagttatgc caaagtgatt aaagaacatg gtactgactt ttttgacaag gatggagtga 361 tgaaagacat cagggcgatt tatcaggttt ataatgcact tcaggagaaa gttcaggcag 421 tgtgtgcaga tgttgaaaag agtgagcgag ttgttgaatc ttgtcaggca gaagtgaaca 481 aattaagaag acaaatcact cagaggaaaa atgaaaagga acaagaaaga agattgcagc 541 aggcagtgtt aagcagacag atgccgtctg aaagcttgga cccagcgttc agtcctcgga 601 tgccgtcctc tgggtttgca gctgaaggca gaagtacact tggagatgca gaggcctcgg 661 atcctcctcc cccttactct gattttcacc caaacaatca agaaagtact ttGAGCCACT 721 CTCGCATGGA AAGGAGTGTC TTTATGCCTC GACCTCAAGC TGTGGGCTCT TCCAATTATG 781 CTTCCACCAG TGCCGGACTG AAGTATCCTG GAAGTGGGGC TGACCTTCCT CCTCCCCAAA 841 GAGCAGCTGG AGACAGTGGT GAGGATTCAG ACGACAGTGA TTATGAAAAT TTGATTGACC 901 CTACAGAGCC TTCTAATAGT GAATACTCAC ATTCAAAGGA TTCTCGACCC ATGGCACATC 961 CCGACGAGGA CCCCAGGAAC ACTCAGACCT CCCAGATTTA CCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGATATCTG GGCGCTCCAC ACCTACCCAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt