Construct: ORF TRCN0000467043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012116.1_s317c1
- Derived from:
- ccsbBroadEn_12998
- DNA Barcode:
- CTCTCAGATTCCCATTCCCCGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNA2D4 (93589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93589 | CACNA2D4 | calcium voltage-gated chann... | NM_172364.5 | 17.9% | 17.9% | 1_2799del |
| 2 | human | 93589 | CACNA2D4 | calcium voltage-gated chann... | XM_011521041.2 | 16.8% | 16% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 678
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta tgactatcag gccatgtgca aaccctcgag tcaccaccac agtgcagccc 121 agcccctggt cagcccaatt tctgccttct tgacggcgac caggtggctg ctgcaggagc 181 tggtgctgtt cctgctggag tggagtgtct ggggctcctg gtacgacaga ggggccgagg 241 ccaaaagtgt cttccatcac tcccacaaac acaagaagca ggacccgctg cagccctgcg 301 acacggagta ccccgtgttc gtgtaccagc cggccatccg ggaggccaac gggatcgtgg 361 agtgcgggcc ctgccagaag gtatttgtgg tgcagcagat tcccaacagt aacctcctcc 421 TCCTGGTGAC AGACCCCACC TGTGACTGCA GCATCTTCCC ACCAGTGCTG CAGGAGGCGA 481 CAGAAGTCAA ATATAATGCC TCTGTCAAAT GTGACCGGAT GCGCTCCCAG AAGCTCCGCC 541 GGCGACCAGA CTCCTGCCAC GCCTTCCATC CAGAGGAGAA TGCCCAGGAC TGCGGCGGCG 601 CCTCGGACAC CTCAGCCTCG CCGCCCCTAC TCCTGCTGCC TGTGTGTGCC TGGGGGCTAC 661 TGCCCCAACT CCTGCGGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 721 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 781 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTCTCAG ATTCCCATTC 841 CCCGGAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt