Construct: ORF TRCN0000467044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006172.1_s317c1
- Derived from:
- ccsbBroadEn_13328
- DNA Barcode:
- GCTTAACGGAGGCCGATTATCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF550 (162972)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467044
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 162972 | ZNF550 | zinc finger protein 550 | XM_011526568.2 | 99.7% | 99.5% | 846A>G;959A>G;1049T>C |
| 2 | human | 162972 | ZNF550 | zinc finger protein 550 | NM_001277090.2 | 97.6% | 97.3% | (many diffs) |
| 3 | human | 162972 | ZNF550 | zinc finger protein 550 | NM_001277091.2 | 97.6% | 97.3% | (many diffs) |
| 4 | human | 162972 | ZNF550 | zinc finger protein 550 | NM_001277092.2 | 97.6% | 97.3% | (many diffs) |
| 5 | human | 162972 | ZNF550 | zinc finger protein 550 | XM_011526567.3 | 97.6% | 97.3% | (many diffs) |
| 6 | human | 162972 | ZNF550 | zinc finger protein 550 | NM_001277093.2 | 32.3% | 30.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1305
- ORF length:
- 1239
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggtgaccttc aaggatgtgg ctgtgacctt tacccgggag gagtggagac 121 agctggacct ggcccagagg accctgtacc gagaggtgat gctggagacc tgtgggcttc 181 tggtttcact agggcatcgg gttcccaaac cagagttggt ccacctgcta gagcatgggc 241 aggagctgtg gatagtgaag agaggcctct cacatgctac ctgtgcaggt gacagagcac 301 aagttcatac cagagagcca accacttacc cgccagtctt atctgagcgg gcctttctcc 361 ggggaagcct gactctggaa tcttcaacct caagtgattc gaggttgggg agagctaggg 421 atgaggaagg gcttttggaa atgcagaaag gtaaagtgac accggagaca gacctccaca 481 aggagaccca tcttgggaaa gtgagccttg aaggtgaagg tttggggaca gatgatggtc 541 tgcactccag ggcattacaa gagtggttat cagcagatgt tctccatgaa tgtgactcac 601 agcaaccagg gaaagacgcc ttgattcatg cagggacaaa cccctacaaa tgcaagcagt 661 gtgggaaagg ttttaacagg aagtggtatc tcgttcgaca tcagagggtt cacactggaa 721 tgaaacccta tgaatgcaat gcatgtggga aagcctttag ccagagctca actctcattc 781 ggcactacct catccacacc ggggagaaac catacaagtg ccttgagtgt gggaaggcct 841 tcaaacgcag gtcgtacctc atgcagcacc atccaatcca cactggagag aaaccctatg 901 agtgtagtca gtgtcgaaag gccttcaccc accggtctac ttttattcgc cataatagga 961 cccacactgg agaaaagccc tttgagtgca aagaatgtga gaaagccttt agcaatagag 1021 cccgcctaat tcaacattac atcatccaca ctGGAGAGAA GCCCTATGAT TGCATGGCAT 1081 GTGGAAAGGC CTTCAGATGC AGCTCAGAAC TCACACAGCA CCAGCGGATT CACACTGGGG 1141 AGAAGCCCTA TGAATGCACC CAGTGTGGGA AAGCCTTTCA CCGGAGCACG TACCTCATTC 1201 AGCACTCTGT CATCCACACT GGGGAGATGC CCTACAAGTG TATCGAGTGT GGGAAGGCCT 1261 TCAAACGCAG GTCACACCTT CTGCAGCACC AAAGGGTCCA TACATGCCCA ACTTTCTTGT 1321 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1381 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1441 GAAAGGACGA GCTTAACGGA GGCCGATTAT CACGACGCGT TAAGTCgaca atcaacctct 1501 ggattacaaa atttgtgaaa gatt