Construct: ORF TRCN0000467092
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006810.1_s317c1
- Derived from:
- ccsbBroadEn_09524
- DNA Barcode:
- TCATCAATTAGCTCTATATGTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLHC1 (130162)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467092
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001135598.1 | 99.8% | 99.5% | 167C>T;910G>A |
| 2 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353781.2 | 99.8% | 99.5% | 167C>T;910G>A |
| 3 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353784.2 | 99.8% | 99.5% | 167C>T;910G>A |
| 4 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353782.2 | 92.2% | 90.7% | (many diffs) |
| 5 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353783.2 | 92.2% | 90.7% | (many diffs) |
| 6 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353779.2 | 85.6% | 85.3% | 167C>T;334_335ins198;712G>A |
| 7 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353786.2 | 85.6% | 85.3% | 167C>T;334_335ins198;712G>A |
| 8 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353787.2 | 85.6% | 85.3% | 167C>T;334_335ins198;712G>A |
| 9 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_152385.4 | 79% | 78.8% | 1_366del;533C>T;1276G>A |
| 10 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353785.2 | 78.2% | 76.5% | (many diffs) |
| 11 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353780.2 | 67.8% | 67.5% | (many diffs) |
| 12 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NR_148541.2 | 23.8% | (many diffs) | |
| 13 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NR_148540.2 | 23% | (many diffs) | |
| 14 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NR_148543.2 | 21.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1458
- ORF length:
- 1392
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gattatcgaa agtaattcct cgaagattca atctcaaata gatcacatca 121 agcagtgtag ggcagagtat gacacaaaag aagtaaaata ttgtactttc tccaaagatc 181 cttcaaaacc tattccaggc atgactcttc aagaatccat gaatctagat gttctcacta 241 aatacatgaa acatcttgaa gataaatatg cagaaattaa acaagctatg ttgataaaat 301 atgtgccagc tcagaggaag gctgatttag atgaagaaat gattgtgtta ttaaaacggc 361 gagatgtagc tgaaaatctg aacaaaaaat tgcagttttg tcatcaaaga ctgcagataa 421 tttcacaggc acttagttca tgggtaaaat ctgatatgag cagcccattt caagactttg 481 tggagcaaat tcagaaaacc aaatatttac aaggtgacca aggcattgtt gaagaactaa 541 tggaagatga tccacgcagg gcaaaagaag ctgaaattat gcttcactac attgaaaggt 601 ttaatgagtt aatctcactt ggtgaatatg aaaaggcagc ttgttatgca gcaaacagtc 661 ctagaagaat tcttcgaaac attggtacaa tgaatacatt taaggctgtt ggaaaaatta 721 gaggaaagcc tcttccatta ctcttatttt ttgaggccct ctttatcaca agtcatgctt 781 ttccatgtcc tgttgatgca gctctaaccc tggaaggaat caaatgtgga ttatcagaaa 841 aacggttaga tttagttacc aattgggtta cacaggaaag actgacattt tctgaggagg 901 ctggggatgt gatttgtgat tatggggagc aggatactta taacaaggcc aagtgcctgg 961 ctttagctca gattatctac agtgaatgtg gcctgcacaa gaaagctatt ctttgcttgt 1021 gtaaacaggg tcagactcat aggGTCATGG AGTACATACA GCAGTTGAAG GACTTTACTA 1081 CCGATGACCT GTTGCAGCTA TTAATGTCAT GTCCCCAAGT TGAATTAATT CAGTGTCTCA 1141 CTAAAGAGTT GAATGAGAAA CAACCATCTT TATCTTTTGG TCTTGCTATA CTTCATCTGT 1201 TCTCTGCAGA CATGAAAAAA GTTGGCATTA AGCTACTTCA AGAAATCAAT AAAGGTGGGA 1261 TAGATGCAGT AGAAAGTCTT ATGATAAATG ATTCCTTTTG CTCCATAGAA AAGTGGCAAG 1321 AAGTGGCAAA TATATGTTCA CAGAATGGCT TTGACAAATT ATCTAATGAC ATCACGTCTA 1381 TTCTTCGATC TCAGGCTGCA GTTACAGAAA TTTCTGAAGA GGATGACGCA GTCAACCTAA 1441 TGGAACATGT GTTTTGGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1501 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCATCAA TTAGCTCTAT 1621 ATGTTCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt