Construct: ORF TRCN0000467100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011254.1_s317c1
- Derived from:
- ccsbBroadEn_03040
- DNA Barcode:
- ATAAATGATAGGGGAGCCCGGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NKIRAS2 (28511)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467100
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28511 | NKIRAS2 | NFKB inhibitor interacting ... | NM_001001349.2 | 100% | 100% | |
| 2 | human | 28511 | NKIRAS2 | NFKB inhibitor interacting ... | NM_001144927.2 | 100% | 100% | |
| 3 | human | 28511 | NKIRAS2 | NFKB inhibitor interacting ... | NM_017595.6 | 100% | 100% | |
| 4 | human | 28511 | NKIRAS2 | NFKB inhibitor interacting ... | XM_005257251.1 | 100% | 100% | |
| 5 | human | 28511 | NKIRAS2 | NFKB inhibitor interacting ... | NM_001144928.2 | 70.6% | 70.6% | 166_167ins168 |
| 6 | human | 28511 | NKIRAS2 | NFKB inhibitor interacting ... | NM_001144929.2 | 50.7% | 31.3% | 165_166ins97;291_292ins185 |
| 7 | mouse | 71966 | Nkiras2 | NFKB inhibitor interacting ... | NM_028024.2 | 90.9% | 98.9% | (many diffs) |
| 8 | mouse | 71966 | Nkiras2 | NFKB inhibitor interacting ... | XM_017314775.1 | 90.9% | 98.9% | (many diffs) |
| 9 | mouse | 71966 | Nkiras2 | NFKB inhibitor interacting ... | XM_017314776.1 | 90.9% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagagctgc aaggtggtcg tgtgtggcca ggcgtctgtg ggcaaaactt 121 caatcctgga gcagcttctg tatgggaacc atgtagtggg ttcggagatg atcgagacgc 181 aggaggacat ctacgtgggc tccattgaga cagaccgggg ggtgcgagag caggtgcgtt 241 tctatgacac ccgggggcTC CGAGATGGGG CCGAACTGCC CCGACACTGC TTCTCTTGCA 301 CTGATGGCTA CGTCCTGGTC TATAGCACAG ATAGCAGAGA GTCTTTTCAG CGTGTGGAGC 361 TGCTCAAGAA GGAGATTGAC AAATCCAAGG ACAAGAAGGA GGTCACCATC GTGGTCCTTG 421 GCAACAAGTG TGACTTACAG GAGCAGCGGC GTGTAGACCC AGATGTGGCT CAGCACTGGG 481 CCAAGTCAGA GAAGGTGAAG CTGTGGGAGG TGTCAGTGGC GGACCGGCGC TCCCTCCTGG 541 AGCCCTTTGT CTACTTGGCC AGCAAGATGA CGCAACCCCA GAGCAAGTCT GCCTTCCCCC 601 TCAGCCGGAA GAACAAGGGC AGCGGCTCCT TGGATGGCTG CCCAACTTTC TTGTACAAAG 661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 781 ACGAATAAAT GATAGGGGAG CCCGGGCAAC GCGTTAAGTC gacaatcaac ctctggatta 841 caaaatttgt gaaagatt