Construct: ORF TRCN0000467108
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003933.1_s317c1
- Derived from:
- ccsbBroadEn_02922
- DNA Barcode:
- ATCGAAGTGTATTTATCACAGTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TES (26136)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467108
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26136 | TES | testin LIM domain protein | NM_015641.4 | 100% | 100% | |
| 2 | human | 26136 | TES | testin LIM domain protein | NM_152829.3 | 97.8% | 97.8% | 0_1ins27 |
| 3 | human | 26136 | TES | testin LIM domain protein | XM_005250258.1 | 79.8% | 79.8% | 0_1ins255 |
| 4 | mouse | 21753 | Tes | testis derived transcript | NM_207176.3 | 86.7% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttaacatgga cctggaaaac aaagtgaaga agatgggctt aggtcacgag caaggatttg 121 gagccccttg tttaaaatgc aaagaaaaat gtgaaggatt cgaactgcac ttctggagaa 181 aaatatgtcg taactgcaag tgtggccaag aagagcatga tgtcctcttg agcaatgaag 241 aggatcgaaa agtgggaaaa ctttttgaag acaccaagta taccactctg attgcaaaac 301 taaagtcaga tggaattccc atgtataaac gcaatgttat gatattgacg aatccagttg 361 ctgccaagaa gaatgtctcc atcaatacag ttacctatga gtgggctcct cctgtccaga 421 atcaagcatt ggccaggcag tacatgcaga tgctacccaa ggaaaagcag ccagtagcag 481 gctcagaggg ggcacagtac cggaagaagc agctggcaaa gcagctccct gcacatgacc 541 aggacccttc aaagtgccat gagttgtctc ccagagaggt gaaggagatg gagcagtttg 601 tgaagaaata taagagcgaa gctctgggag taggagatgt caaacttccc tgtgagatgg 661 atgcccaagg ccccaaacaa atgaacattc ctggagggga tagaagcacc ccagcagcag 721 tgggggccat ggaggacaaa tctgctgagc acaaaagaac tcaatattcc tgctattgct 781 gcaaactgag tatgaaagaa ggtgacccag ccatctatgc cgaaagggct ggctatgata 841 aactgtggca cccagcttgt tttgtctgca gcacctgcca tgaactcctg gttgacatga 901 tttatttttg gaagaatgag aagctatact gtggcagaca ttactgtgac agcgagaaac 961 cccgatgtgc tggctgtgac gagctgatat tcagcaatga gtatacccag gcagaaaacc 1021 agaattggca cctgaaacac ttctgctgct ttgactgtga tagcattcta gctggggaga 1081 tatacgtgat ggtcaatgac aagcccgtgt gcaagccctg ctatgtgaag aatcacgctg 1141 tggtgtgtca aggatgccac aatgccatcg acccagaagt gcagcgggtg acctataaca 1201 atttcagctg gcatgcaTCC ACAGAGTGCT TTCTGTGCTC TTGCTGCAGC AAATGCCTCA 1261 TTGGGCAGAA GTTCATGCCA GTAGAAGGGA TGGTTTTCTG TTCAGTGGAA TGTAAGAAGA 1321 GGATGTCTTA GGAGGAGGGC ACCCAGAAGT ATCGAGCCAT AGCTATCCAA AGTGGTCTGC 1381 ATTTCTACTG TAAAATGCAA TTTGAAAAAA ATAAAACGCA AAAAAAGAAA CTGTAAAAAA 1441 AAAAAAAAAA AACTCGAGGC ATCTATGTCG GGTGCGGAGA AAGAGGTAAT GAAATGGCAC 1501 ATGGTCATAG CTGTTTCCTG ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 1561 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 1621 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATCGA AGTGTATTTA 1681 TCACAGTGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt