Construct: ORF TRCN0000467139
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001476.1_s317c1
- Derived from:
- ccsbBroadEn_13040
- DNA Barcode:
- CTGAATGACTCAAGCGAATCGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL29 (114818)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467139
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114818 | KLHL29 | kelch like family member 29 | XM_011532501.2 | 57.9% | 57.6% | (many diffs) |
| 2 | human | 114818 | KLHL29 | kelch like family member 29 | NM_052920.2 | 57.4% | 57.4% | 1_597del;2097C>T;2107_2625del |
| 3 | human | 114818 | KLHL29 | kelch like family member 29 | XM_006711929.3 | 57.4% | 57.4% | 1_597del;2097C>T;2107_2625del |
| 4 | human | 114818 | KLHL29 | kelch like family member 29 | XM_017003264.2 | 57.4% | 57.4% | 1_597del;2097C>T;2107_2625del |
| 5 | mouse | 208439 | Klhl29 | kelch-like 29 | NM_001164493.1 | 52.7% | 56.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1575
- ORF length:
- 1509
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aggccactac tcgctccctc agccgccctc tcagccactg agcagcgtgg 121 tggtcaacat gcctgcccag gccctgtatg ccagccctca gcccctggcc gtgtccacac 181 tgcccggtgt ggggcaggtg gcccgcccag gacccaccgc tgtgggcaac ggccacatgg 241 cagggcccct gctgcctcca ccgccgccag cccagccgtc cgccactctc cccagtggtg 301 cccctgccac caatgggccc cccacaaccg actcggccca cgggctgcag atgctgcgga 361 ccattggcgt ggggaagtat gagttcaccg acccggggca ccccagagaa atgttgaagg 421 aattgaacca gcaacgcaga gcgaaagcgt ttacagacct gaaaattgtt gttgaaggca 481 gagagtttga agtccaccaa aatgttctag cttcctgcag cttgtatttc aaggacctga 541 ttcaaaggtc cgtgcaagac agcggccagg gcggccggga gaagctggag ctcgtcctgt 601 cgaacctgca ggcagacgtc ctggagttgc tgctggagtt tgtctacacg ggctccctgg 661 tcatcgactc ggccaacgcc aagacactgc tggaggcggc cagcaagttc cagttccaca 721 ccttctgcaa agtctgcgtg tcctttctcg agaagcagct gacggccagc aactgcctgg 781 gcgtgctggc catggccgag gccatgcagt gcagcgagct ctaccacatg gccaaggcct 841 tcgcgctgca gatcttcccc gaggtggccg cccaggagga gatcctcagc atctccaagg 901 acgacttcat cgcctacgtc tccaacgaca gcctcaacac caaggctgag gagctggtgt 961 acgagacagt catcaagtgg atcaagaagg accccgcgac acgcacacag tacgcggctg 1021 agctcctggc cgtggtccgc ctccccttca tccaccccag ctacctgctc aatgtggttg 1081 acaatgaaga gctgatcaag tcatcagaag cctgccggga ccTGGTGAAC GAGGCCAAAC 1141 GCTACCATAT GCTGCCCCAC GCCCGCCAGG AGATGCAGAC GCCCCGAACC CGGCCGCGCC 1201 TCTCTGCAGG TGTGGCTGAG GTCATCGTCT TGGTTGGGGG CCGTCAGATG GTGGGGATGA 1261 CCCAGCGCTC GCTGGTGGCC GTCACCTGCT GGAACCCGCA GAACAACAAG TGGTACCCCT 1321 TGGCCTCGCT GCCCTTCTAT GACCGCGAGT TCTTCAGTGT AGTGAGTGCA GGGGACAACA 1381 TCTACCTCTC AGGTGGGATG GAATCAGGGG TGACGCTGGC TGATGTCTGG TGCTACATGT 1441 CCCTGCTTGA TAACTGGAAC CTCGTCTCCA GAATGACAGT CCCCCGCTGT CGGCACAATA 1501 GCCTCGTCTA CGATGGGAAG ATTTACACCC TCGGGGGACT TGGCGTGGCA GGCAACGTGG 1561 ACCATGTGGA GAGGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTGAATGACT CAAGCGAATC 1741 GATCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt