Construct: ORF TRCN0000467194
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000549.1_s317c1
- Derived from:
- ccsbBroadEn_13149
- DNA Barcode:
- CAGAGTACTCCGAGACTACAGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C2orf73 (129852)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467194
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 129852 | C2orf73 | chromosome 2 open reading f... | NM_001369403.1 | 99.5% | 98.7% | 398C>T;461G>C |
| 2 | human | 129852 | C2orf73 | chromosome 2 open reading f... | XM_005264134.3 | 99.5% | 98.7% | 398C>T;461G>C |
| 3 | human | 129852 | C2orf73 | chromosome 2 open reading f... | XM_011532513.3 | 99.5% | 98.7% | 398C>T;461G>C |
| 4 | human | 129852 | C2orf73 | chromosome 2 open reading f... | NM_001369401.1 | 72.1% | 71.6% | 1_189del;587C>T;650G>C |
| 5 | human | 129852 | C2orf73 | chromosome 2 open reading f... | NM_001100396.2 | 57.6% | 57.1% | 1_363del;761C>T;824G>C |
| 6 | human | 129852 | C2orf73 | chromosome 2 open reading f... | XM_024452688.1 | 57.6% | 57.1% | 1_363del;761C>T;824G>C |
| 7 | human | 129852 | C2orf73 | chromosome 2 open reading f... | XM_024452687.1 | 47.9% | 47.5% | 1_537del;935C>T;998G>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 567
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaaaagcct tcttgtggaa tagtaccact tgcctctcct ggtacatcag 121 cagaacttca aaacaatttt atagaataca tctcttttat acatcaatat gatgccagaa 181 aaactccgaa tgagcctctc cagggaaaga gacacggagc ttttgtacag agagagataa 241 aaccaggcag taggccaaca gttccTAAAG GAGCAGAGGT ATTACTGAAC ACTCCAGGGT 301 CACGTTCATC AGAACAGTCC AAAAAAACAG AGAAAGGAAA CTCAGCGGAA AGCAGAATGA 361 TCTCACCAGG TCTCTGCCAA CAAAATTCTC AGGAGCTGTT AGAGCCTAAA ACTCACTTAT 421 CAGAAACAGA CGTCAGACAG GCAGCCAAGG CATGCCCCAG CACTCTTGAG AGCAGAGAAA 481 AGACCTCAGG CGCCACTCAA ACAACTGTAG GAGATGCTCT TTTCACTACG CACAAGCCTT 541 TAAATCCACC AATTAAAAAA TCAGAATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACAGAGTAC 721 TCCGAGACTA CAGGTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt